HtrA

• Description: membrane-anchored protein quality control protease, serine protease Do
  
  

Gene name	htrA
Synonyms	ykdA
Essential	no
Product	serine protease Do
Function	protein quality control
Gene expression levels in SubtiExpress: htrA
Metabolic function and regulation of this protein in SubtiPathways: htrA
MW, pI	47 kDa, 4.699
Gene length, protein length	1347 bp, 449 aa
Immediate neighbours	ykcC, proG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteolysis, heat shock proteins, membrane proteins

This gene is a member of the following regulons

CssR regulon

The gene

Basic information

• Locus tag: BSU12900

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • required for folding of the secreted protein YqxI PubMed

• Protein family: PDZ (DHR) domain (according to Swiss-Prot)

• Paralogous protein(s): HtrC

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)
  • extracellular (signal peptide) PubMed
  • randomly distributed in foci throughout the cell surface PubMed

Database entries

• Structure:

• UniProt: O34358

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: htrA (according to DBTBS)

• Expression browser: htrA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions of secretion stress (CssR) PubMed
  • induced by rhamnolipids PubMed

• Regulatory mechanism:
  • CssR: transcription activation PubMed

• Additional information:
  • HtrA is subject to degradation by WprA and other extracellular proteases PubMed

Biological materials

• Mutant:
  • 1A933 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Ross E Dalbey, Peng Wang, Jan Maarten van Dijl
Membrane proteases in the bacterial protein secretion and quality control pathway.
Microbiol Mol Biol Rev: 2012, 76(2);311-30
[PubMed:22688815] [WorldCat.org] [DOI] (I p)

Tim Clausen, Markus Kaiser, Robert Huber, Michael Ehrmann
HTRA proteases: regulated proteolysis in protein quality control.
Nat Rev Mol Cell Biol: 2011, 12(3);152-62
[PubMed:21326199] [WorldCat.org] [DOI] (I p)