Difference between revisions of "CadA"

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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
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* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
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* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
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* '''Structure:'''
 
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** ** [http://www.pdb.org/pdb/explore/explore.do?structureId=4BBJ 4BBJ] (CopA from ''Legionella pneumophila'', 32% identity, 67% similarity) {{PubMed|24317491}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O32219 O32219]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O32219 O32219]
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=References=
 
=References=
  
<pubmed>15948947,12779235,,11934502 , </pubmed>
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<pubmed>15948947,12779235,24317491,11934502 , </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 13:34, 8 January 2014

  • Description: cadmium transporting ATPase, resistance to cadmium

Gene name cadA
Synonyms yvgW
Essential no
Product cadmium transporting ATPase
Function cadmium export
Gene expression levels in SubtiExpress: cadA
Metabolic function and regulation of this protein in SubtiPathways:
metal ion homeostasis
MW, pI 75 kDa, 5.998
Gene length, protein length 2106 bp, 702 aa
Immediate neighbours bdbD, copA
Sequences Protein DNA DNA_with_flanks
Genetic context
YvgW context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CadA expression.png















Categories containing this gene/protein

transporters/ other, resistance against toxic metals, membrane proteins

This gene is a member of the following regulons

CzrA regulon

The gene

Basic information

  • Locus tag: BSU33490

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + H2O + Cd2+(In) = ADP + phosphate + Cd2+(Out) (according to Swiss-Prot)
  • Protein family: Type IB subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
    • ** 4BBJ (CopA from Legionella pneumophila, 32% identity, 67% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
    • induced by toxic metal ions (Zn(II), Cd(II), Co(II), Ni(II) and Cu(II)) (CzrA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

Your additional remarks

References

Magnus Andersson, Daniel Mattle, Oleg Sitsel, Tetyana Klymchuk, Anna Marie Nielsen, Lisbeth Birk Møller, Stephen H White, Poul Nissen, Pontus Gourdon
Copper-transporting P-type ATPases use a unique ion-release pathway.
Nat Struct Mol Biol: 2014, 21(1);43-8
[PubMed:24317491] [WorldCat.org] [DOI] (I p)

Charles M Moore, Ahmed Gaballa, Monica Hui, Rick W Ye, John D Helmann
Genetic and physiological responses of Bacillus subtilis to metal ion stress.
Mol Microbiol: 2005, 57(1);27-40
[PubMed:15948947] [WorldCat.org] [DOI] (P p)

Ahmed Gaballa, John D Helmann
Bacillus subtilis CPx-type ATPases: characterization of Cd, Zn, Co and Cu efflux systems.
Biometals: 2003, 16(4);497-505
[PubMed:12779235] [WorldCat.org] [DOI] (P p)

I M Solovieva, K D Entian
Investigation of the yvgW Bacillus subtilis chromosomal gene involved in Cd(2+) ion resistance.
FEMS Microbiol Lett: 2002, 208(1);105-9
[PubMed:11934502] [WorldCat.org] [DOI] (P p)