mmgD

168

2-methylcitrate synthase

Locus: BSU_24140

Molecular weight: 41.95 kDa

Isoelectric point: 6.2

Protein length: 372 aa

Gene length: 1119 bp

Function: mother cell metabolism

Product: 2-methylcitrate synthase

Essential: no

E.C.: 2.3.3.5

Synonyms: mmgD, yqiO, prpC

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Metabolism, Amino acid/ nitrogen metabolism, Utilization of amino acids, Utilization of branched-chain amino acids

Lifestyles, Sporulation, Sporulation proteins, Sporulation proteins/ other

List of homologs in different organisms, belongs to COG0372 (Galperin et al., 2021)

Gene

Coordinates: 2,509,654  2,510,772

The protein

Catalyzed reaction/ biological activity

propionyl-CoA + H₂O + oxaloacetate --> 2-methylcitrate + CoA PubMed
does also have (albeit weaker) citrate synthase activity PubMed
acetyl-CoA + H₂O + oxaloacetate --> citrate + CoA + H⁺ (according to UniProt)

Protein family

citrate synthase family (with CitA and CitZ, according to UniProt)

Structure

3HWK (PDB) (from Mycobacterium tuberculosis, 38% identity) PubMed
P45858 (AlphaFold)

Paralogous protein(s)

CitZ, CitA

Expression and Regulation

Operons

Genes: mmgA-mmgB-mmgC-mmgD-mmgE-mmgF

Description: PubMed

Regulation

expressed early during sporulation in the mother cell (SigE) PubMed
strongly expressed during oligotrophic growth PubMed

Regulatory mechanism

CcpA: repression, PubMed, in ccpA regulon

Sigma factors

SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

BKE24140 (mmgD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCCTCAACCTCCGTTT,  downstream forward: _UP4_AAATCATGAAAGGAGCTGGT
BKK24140 (mmgD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCCTCAACCTCCGTTT,  downstream forward: _UP4_AAATCATGAAAGGAGCTGGT

References

Loading