Difference between revisions of "MleN"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23560 mleN]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23560 mleN]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mleN mleN]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 7.249   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 7.249   

Revision as of 11:08, 7 January 2014

  • Description: malate-H+/Na+-lactate antiporter

Gene name mleN
Synonyms yqkI
Essential no
Product malate-H+/Na+-lactate antiporter
Function malate uptake
Gene expression levels in SubtiExpress: mleN
Metabolic function and regulation of this protein in SubtiPathways:
mleN
MW, pI 50 kDa, 7.249
Gene length, protein length 1404 bp, 468 aa
Immediate neighbours mleA, ansB
Sequences Protein DNA DNA_with_flanks
Genetic context
MleN context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MleN expression.png



















Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

AnsR regulon, CcpA regulon

The gene

Basic information

  • Locus tag: BSU23560

Phenotypes of a mutant

abrupt arrest in the mid-logarithmic phase of growth on malate when low concentrations of protonophore were present PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: nhaC Na(+)/H(+) antiporter family (according to Swiss-Prot)
  • Paralogous protein(s): NhaC

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:GP1460 (mleN::spc), available in Stülke lab
  • Expression vector:
  • lacZ fusion: pGP388 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References