MrpD

• Description: H+ transporter subunit of the Na+/H+ antiporter, multiple resistance and pH homeostasis
  
  

Gene name	mrpD
Synonyms	yufD
Essential	yes PubMed
Product	Na+/H+ antiporter subunit
Function	sodium export
Gene expression levels in SubtiExpress: mrpD
Interactions involving this protein in SubtInteract: MrpD
MW, pI	53 kDa, 9.488
Gene length, protein length	1479 bp, 493 aa
Immediate neighbours	mrpC, mrpE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), essential genes, membrane proteins

The gene

Basic information

• Locus tag: BSU31630

Phenotypes of a mutant

• essential PubMed
• a mrpD mutant is viable at pH7.4 and sodium concentrations below 80 mM PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: H+ transporter subunit of the Na+/H+ antiporter PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • MrpA-MrpB-MrpC-MrpD-MrpE-MrpF-MrpG (complex)

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O05229

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mrpA-mrpB-mrpC-mrpD-mrpE-mrpF-mrpG PubMed

• Expression browser: mrpD PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Vamsi K Moparthi, Brijesh Kumar, Yusra Al-Eryani, Eva Sperling, Kamil Górecki, Torbjörn Drakenberg, Cecilia Hägerhäll
Functional role of the MrpA- and MrpD-homologous protein subunits in enzyme complexes evolutionary related to respiratory chain complex I.
Biochim Biophys Acta: 2014, 1837(1);178-85
[PubMed:24095649] [WorldCat.org] [DOI] (P p)

Vamsi K Moparthi, Brijesh Kumar, Cecilie Mathiesen, Cecilia Hägerhäll
Homologous protein subunits from Escherichia coli NADH:quinone oxidoreductase can functionally replace MrpA and MrpD in Bacillus subtilis.
Biochim Biophys Acta: 2011, 1807(4);427-36
[PubMed:21236240] [WorldCat.org] [DOI] (P p)

Yusuke Kajiyama, Masato Otagiri, Junichi Sekiguchi, Toshiaki Kudo, Saori Kosono
The MrpA, MrpB and MrpD subunits of the Mrp antiporter complex in Bacillus subtilis contain membrane-embedded and essential acidic residues.
Microbiology (Reading): 2009, 155(Pt 7);2137-2147
[PubMed:19389778] [WorldCat.org] [DOI] (P p)

Yusuke Kajiyama, Masato Otagiri, Junichi Sekiguchi, Saori Kosono, Toshiaki Kudo
Complex formation by the mrpABCDEFG gene products, which constitute a principal Na+/H+ antiporter in Bacillus subtilis.
J Bacteriol: 2007, 189(20);7511-4
[PubMed:17693497] [WorldCat.org] [DOI] (P p)

M Ito, A A Guffanti, W Wang, T A Krulwich
Effects of nonpolar mutations in each of the seven Bacillus subtilis mrp genes suggest complex interactions among the gene products in support of Na(+) and alkali but not cholate resistance.
J Bacteriol: 2000, 182(20);5663-70
[PubMed:11004162] [WorldCat.org] [DOI] (P p)

M Ito, A A Guffanti, B Oudega, T A Krulwich
mrp, a multigene, multifunctional locus in Bacillus subtilis with roles in resistance to cholate and to Na+ and in pH homeostasis.
J Bacteriol: 1999, 181(8);2394-402
[PubMed:10198001] [WorldCat.org] [DOI] (P p)