Difference between revisions of "RelA"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* requirement for valine {{PubMed|24163341}}
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* a ''[[relA]] [[yjbM]] [[ywaC]]'' triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of ''[[guaB]]'' or inactivation of ''[[codY]]'' {{PubMed|24163341}}
  
 
=== Database entries ===
 
=== Database entries ===
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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
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* '''[[Domains]]:'''  
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** N-terminal ppGpp hydrolase domain {{PubMed|24163341}}
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** central ppGpp synthetase domain {{PubMed|24163341}}
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
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* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=relA_2820529_2822733_-1 relA] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=relA_2820529_2822733_-1 relA] {{PubMed|22383849}}
  
* '''Sigma factor:'''
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* '''[[Sigma factor]]:'''
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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=References=
 
=References=
 
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<pubmed>11948165,9383190,10209741,13129942,, 19447912 13129942  12372825  12081964  11948165 12419222 23028324 24163341</pubmed>
<pubmed>11948165,9383190,10209741,13129942,, 19447912 13129942  12372825  12081964  11948165 12419222 23028324 </pubmed>
 
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 11:47, 22 December 2013

  • Description: GTP pyrophosphokinase (stringent response)

Gene name relA
Synonyms
Essential no
Product GTP pyrophosphokinase
Function synthesis and degradation of (p)ppGpp,
triggering the stringent response
Gene expression levels in SubtiExpress: relA
Metabolic function and regulation of this protein in SubtiPathways:
Purine salvage, Nucleotides (regulation), Murein recycling
MW, pI 84 kDa, 6.964
Gene length, protein length 2202 bp, 734 aa
Immediate neighbours yrvI, apt
Sequences Protein DNA DNA_with_flanks
Genetic context
RelA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RelA expression.png















Categories containing this gene/protein

metabolism of signalling nucleotides

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU27600

Phenotypes of a mutant

  • requirement for valine PubMed
  • a relA yjbM ywaC triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of guaB or inactivation of codY PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate (according to Swiss-Prot)
  • Protein family: relA/spoT family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

Your additional remarks

References