Difference between revisions of "OdhA"

From SubtiWiki
Jump to: navigation, search
Line 80: Line 80:
 
* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
 
** phosphorylated on Arg-66 and Arg-621 {{PubMed|22517742}}
 
** phosphorylated on Arg-66 and Arg-621 {{PubMed|22517742}}
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
Line 113: Line 113:
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=odhA_2108774_2111608_-1 odhA] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=odhA_2108774_2111608_-1 odhA] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigA]] {{PubMed|1508153}}  
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|1508153}}  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
Line 147: Line 147:
 
<pubmed> 10672230</pubmed>
 
<pubmed> 10672230</pubmed>
 
==Original publications==
 
==Original publications==
<pubmed>2500417,20933603,1508153, 11976317 12850135 22517742 22900538 </pubmed>
+
<pubmed>2500417,20933603,1508153, 11976317 12850135 22517742 22900538 24263382 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 09:54, 17 December 2013

  • Description: 2-oxoglutarate dehydrogenase (E1 subunit)

Gene name odhA
Synonyms citK
Essential no
Product 2-oxoglutarate dehydrogenase (E1 subunit)
Function TCA cycle
Gene expression levels in SubtiExpress: odhA
Interactions involving this protein in SubtInteract: OdhA
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 105 kDa, 5.871
Gene length, protein length 2823 bp, 941 aa
Immediate neighbours odhB, yojO
Sequences Protein DNA DNA_with_flanks
Genetic context
OdhA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
OdhA expression.png















Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

  • Locus tag: BSU19370

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 (according to Swiss-Prot)
  • Protein family: 2-oxoacid dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
    • phosphorylated on Arg-66 and Arg-621 PubMed
  • Effectors of protein activity:

Database entries

  • Structure: 2JGD (from Escherichia coli, 38% identity, 55% similarity) PubMed
  • KEGG entry: [3]

Additional information

  • extensive information on the structure and enzymatic properties of 2-oxoglutarate dehydrogenase can be found at Proteopedia

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications