Difference between revisions of "GroEL"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
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** phosphorylated on ser/ thr/ tyr [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed] | ** phosphorylated on ser/ thr/ tyr [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed] | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=groEL_650234_651868_1 groEL] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=groEL_650234_651868_1 groEL] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigA]] {{PubMed|1350776}} | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|1350776}} |
* '''Regulation:''' induced by heat shock ([[HrcA]]) {{PubMed|1350777,8576042}} | * '''Regulation:''' induced by heat shock ([[HrcA]]) {{PubMed|1350777,8576042}} | ||
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* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | ** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP1000 available in [[Stülke]] lab | + | ** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP1000 available in [[Jörg Stülke]]'s lab |
| − | ** for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP1002 available in [[Stülke]] lab | + | ** for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP1002 available in [[Jörg Stülke]]'s lab |
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
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=References= | =References= | ||
| − | + | <pubmed>9303302,1350777,12082092,,17726680 16493705 9303302,1350776 ,8576042 7592421 22517742 22268681 24263382</pubmed> | |
| − | <pubmed>9303302,1350777,12082092,,17726680 16493705 9303302,1350776 ,8576042 7592421 22517742</pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 07:33, 16 December 2013
- Description: chaperonin and co-repressor for HrcA
| Gene name | groEL |
| Synonyms | |
| Essential | yes PubMed |
| Product | chaperonin, co-repressor for HrcA |
| Function | protein folding and re-folding |
| Gene expression levels in SubtiExpress: groEL | |
| Interactions involving this protein in SubtInteract: GroEL | |
| Metabolic function and regulation of this protein in SubtiPathways: Stress | |
| MW, pI | 57 kDa, 4.531 |
| Gene length, protein length | 1632 bp, 544 aa |
| Immediate neighbours | groES, ydzT/1 |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
chaperones/ protein folding, heat shock proteins, essential genes, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU06030
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: acts as co-repressor for HrcA
- Protein family: chaperonin (HSP60) family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization:
- cytoplasm (according to Swiss-Prot)
- recruited to the membrane after ethanol stress PubMed
Database entries
- Structure:
- UniProt: P28598
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1000 available in Jörg Stülke's lab
- for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP1002 available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Wolfgang Schumann, Bayreuth University, Germany Homepage
Your additional remarks
References
