PgdS

• Description: gamma-DL-glutamyl hydrolase
  
  

Gene name	pgdS
Synonyms	ywtD
Essential	no
Product	gamma-DL-glutamyl hydrolase
Function	polyglutamic acid degradation
Gene expression levels in SubtiExpress: pgdS
MW, pI	45 kDa, 8.52
Gene length, protein length	1239 bp, 413 aa
Immediate neighbours	ywtE, capE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

capsule biosynthesis and degradation

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

• Locus tag: BSU35860

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: nlpC/p60 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization: secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed

Database entries

• Structure:

• UniProt: P96740

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pgdS PubMed

• Expression browser: pgdS PubMed

• Sigma factor: SigD PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion: available in Daniel Kearns's lab PubMed

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Viola Scoffone, Daniele Dondi, Ginevra Biino, Giovanni Borghese, Dario Pasini, Alessandro Galizzi, Cinzia Calvio
Knockout of pgdS and ggt genes improves γ-PGA yield in B. subtilis.
Biotechnol Bioeng: 2013, 110(7);2006-12
[PubMed:23335395] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Masakuni Serizawa, Hiroki Yamamoto, Hirotake Yamaguchi, Yasutaro Fujita, Kazuo Kobayashi, Naotake Ogasawara, Junichi Sekiguchi
Systematic analysis of SigD-regulated genes in Bacillus subtilis by DNA microarray and Northern blotting analyses.
Gene: 2004, 329;125-36
[PubMed:15033535] [WorldCat.org] [DOI] (P p)

Takao Suzuki, Yasutaka Tahara
Characterization of the Bacillus subtilis ywtD gene, whose product is involved in gamma-polyglutamic acid degradation.
J Bacteriol: 2003, 185(7);2379-82
[PubMed:12644511] [WorldCat.org] [DOI] (P p)