Difference between revisions of "PtsG"
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13890 ptsG] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13890 ptsG] | ||
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− | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PtsG PtsG] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism], [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism], [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]''' |
Revision as of 16:07, 11 November 2013
- Description: trigger enzyme: major glucose permease of the PTS, EIICBA(Glc) and control of GlcT activity
Gene name | ptsG |
Synonyms | ptsX, crr |
Essential | no |
Product | trigger enzyme: glucose-specific enzyme IICBA component of the PTS |
Function | glucose transport and phosphorylation, control of GlcT activity |
Gene expression levels in SubtiExpress: ptsG | |
Interactions involving this protein in SubtInteract: PtsG | |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Sugar catabolism | |
MW, pI | 75,3 kDa, 5.40 |
Gene length, protein length | 2097 bp, 699 amino acids |
Immediate neighbours | glcT, ptsH |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
phosphotransferase systems, carbon core metabolism, transcription factors and their control, trigger enzyme, membrane proteins, phosphoproteins
This gene is a member of the following regulons
GlcT regulon, stringent response
The gene
Basic information
- Locus tag: BSU13890
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: transport and phosphorylation of glucose, receives a phosphate from HPr at the IIA domain (His-620), the phosphate group is then transferred to the IIB domain (Cys-461) an finally to the incoming glucose. In the absence of glucose, PtsG phosphorylates and thereby inactivates the transcriptional antiterminator GlcT.
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- 11x transmembrane domain (16–36, 89–109, 139–159, 180–200, 233–253, 283–303, 313–333, 338–358, 365–385, 388–408)
- PTS EIIC domain ( 1-424)
- PTS EIIB domain (439–520)
- PTS EIIA domain (568–672)
- Modification: transient phosphorylation (HPr-dependent) on His-620, then internal phosphotransfer from His-620 to Cys-461
- Cofactor(s):
- Effectors of protein activity:
- Localization: membrane protein PubMed
Database entries
- UniProt: P20166
- KEGG entry: [3]
- E.C. number: 2.7.1.69
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- transcriptional antitermination via the GlcT-dependent RNA switch PubMed
- stringent response: due to presence of guanine at +1 position of the transcript PubMed
- Additional information:
Biological materials
- Mutants: all available in Jörg Stülke's lab
- Expression vector:
- pGP123 (domains BA, in pWH844), available in Jörg Stülke's lab
- pGP141 (domains BA, mut: H620D, in pWH844), available in Jörg Stülke's lab
- pGP428 (EIIB, in pWH844), available in Jörg Stülke's lab
- pGP437(EIIA in pGP570, with thrombin cleavage site), available in Jörg Stülke's lab
- lacZ fusion:
- pGP34 (pAC5) PubMed, available in Jörg Stülke's lab
- pGP66 (pAC7) PubMed, available in Jörg Stülke's lab
- pGP606 (mutant terminator, pAC6), available in Jörg Stülke's lab
- pGP532 (pAC7), available in Jörg Stülke's lab
- series of promoter deletions are available in pAC5 and pAC6, available in Jörg Stülke's lab
- series of RAT mutants are available in pAC6, available in Jörg Stülke's lab
- GFP fusion:
- Antibody:
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany Homepage
Your additional remarks
References
Reviews
Original publications