Difference between revisions of "PBP137"

From SubtiWiki
Jump to: navigation, search
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
 
[[File:Map_pBP137.png|250px|thumb|right|'''pBP137: click to enlarge''']]
 
[[File:Map_pBP137.png|250px|thumb|right|'''pBP137: click to enlarge''']]
 
+
[[File:PBP137_MCS.png|250px|thumb|right|'''pBP137 cloning region: click to enlarge''']]
  
 
Vector for the construction of translational ''gfp'' fusions that can be integrated at the ''amyE'' site
 
Vector for the construction of translational ''gfp'' fusions that can be integrated at the ''amyE'' site

Latest revision as of 16:15, 29 October 2013

pBP137: click to enlarge
pBP137 cloning region: click to enlarge

Vector for the construction of translational gfp fusions that can be integrated at the amyE site

Does not replicate in B. subtilis, integrates into the amyE site

Constructed in Fabian Commichau's lab

  • in E. coli: ampicillin resistance
  • in B. subtilis: chloramphenicol resistance

sequencing primers:

  • KG307: 5’-CACCTTCACCCTCTCCACTGACAG -3’
  • PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘