Difference between revisions of "PWH844"
Raphael2215 (talk | contribs) |
|||
| (One intermediate revision by one other user not shown) | |||
| Line 17: | Line 17: | ||
*'''SH71:''' 5‘-'''AACGGTGGTA'''TATCCAGTG-3‘ | *'''SH71:''' 5‘-'''AACGGTGGTA'''TATCCAGTG-3‘ | ||
*'''PQE9-R348:''' 5’-TTGGGATATATC'''AACGGTGGTA'''-3’ | *'''PQE9-R348:''' 5’-TTGGGATATATC'''AACGGTGGTA'''-3’ | ||
| − | *<font color="#99E500">''' | + | *<font color="#99E500">'''JK37'''</font>: 5’-GGATAACAATTATAATAGATTCAATTGTGAGCGGATAAC-3‘ |
<pubmed> 9023219 </pubmed> | <pubmed> 9023219 </pubmed> | ||
| + | == Return to [[Plasmids]] == | ||
Latest revision as of 14:23, 8 October 2013
vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein
the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Hillen lab
in E. coli: ampicillin resistance
sequencing primer:
- JS39: 5’-TCTATCAACAGGAGTCCAAGC-3‘
- SH71: 5‘-AACGGTGGTATATCCAGTG-3‘
- PQE9-R348: 5’-TTGGGATATATCAACGGTGGTA-3’
- JK37: 5’-GGATAACAATTATAATAGATTCAATTGTGAGCGGATAAC-3‘
F Schirmer, S Ehrt, W Hillen
Expression, inducer spectrum, domain structure, and function of MopR, the regulator of phenol degradation in Acinetobacter calcoaceticus NCIB8250.
J Bacteriol: 1997, 179(4);1329-36
[PubMed:9023219]
[WorldCat.org]
[DOI]
(P p)
