Difference between revisions of "YwrD"

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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1575 bp, 525 aa  
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1575 bp, 525 aa  
 
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|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywrE]]'', ''[[ywrC]]''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywrE]]'', ''[[chrS]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36100 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36100 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36100 DNA_with_flanks]
 
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36100 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36100 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36100 DNA_with_flanks]

Revision as of 09:20, 2 September 2013

  • Description: unknown

Gene name ywrD
Synonyms
Essential no
Product unknown
Function unknown
Gene expression levels in SubtiExpress: ywrD
MW, pI 57 kDa, 5.451
Gene length, protein length 1575 bp, 525 aa
Immediate neighbours ywrE, chrS
Sequences Protein DNA DNA_with_flanks
Genetic context
YwrD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YwrD expression.png
























Categories containing this gene/protein

proteins of unknown function

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

  • Locus tag: BSU36100

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl amino acid (according to Swiss-Prot), however, all evidence suggests this is a misannotation PubMed
  • Protein family: gamma-glutamyltransferase family (according to Swiss-Prot), , however, all evidence suggests this is a misannotation PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

The gene is annotated in KEGG as an ortholog of gamma-glutamyltranspeptidase EC 2.3.2.2, This enzyme is necessary to utilize glutathione (GSH) as the sulfur source. No EC annotation for this gene is available in Swiss-Prot/MetaCyc. It has been shown by Minami et al. (PubMed) that ywrD mutant grows well on minimal media supplied with GSH as the sole sulfur source. In addition, His-tag purified ywrD cannot hydrolyze GSH. PubMed


Expression and regulation

  • Sigma factor:
  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hiromichi Minami, Hideyuki Suzuki, Hidehiko Kumagai
Gamma-glutamyltranspeptidase, but not YwrD, is important in utilization of extracellular glutathione as a sulfur source in Bacillus subtilis.
J Bacteriol: 2004, 186(4);1213-4
[PubMed:14762019] [WorldCat.org] [DOI] (I p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)