defA

168

formylmethionine deformylase, binds and inhibits PtkA during oxidative stress

Locus: BSU_15720

Molecular weight: 17.63 kDa

Isoelectric point: 4.73

Protein length: 160 aa

Gene length: 483 bp

Function: translation

Product: formylmethionine deformylase

Essential: no

E.C.: 3.5.1.88

Synonyms: defA, yloK, def

Genomic Context

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List of homologs in different organisms, belongs to COG0242 (Galperin et al., 2021)

Gene

Coordinates: 1,646,512  1,646,994

Phenotypes of a mutant

• a ''defA defB double mutant is not viable unless the cells acquire suppressor mutations in fmt, glyA or folD'' PubMed

The protein

Catalyzed reaction/ biological activity

• H₂O + N-terminal N-formyl-L-methionyl-[peptide] --> formate + N-terminal L-methionyl-[peptide] (according to UniProt)
• binds and inhibits PtkA during oxidative stress PubMed

Protein family

• polypeptide deformylase family (with DefB, according to UniProt)

Structure

• 1WS0 (PDB) (from B.cereus, 67% identity) PubMed
• P94462 (AlphaFold)

Paralogous protein(s)

• DefB

Expression and Regulation

Operons

• Genes: defA-fmt-yloM-yloN

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: yloC-remA-gmk-yloH-coaBC-priA-defA-fmt-yloM-yloN

  Description

  • PubMed

Biological materials

Mutant

• BKE15720 (defA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTTGTTACCCTCCAAGA,  downstream forward: _UP4_CTAGCGGATATGGAAGGATG
• BKK15720 (defA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTTGTTACCCTCCAAGA,  downstream forward: _UP4_CTAGCGGATATGGAAGGATG

References

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