cmoJ

168

flavin-dependent monooxygenase, required for the conversion of S-methyl-cysteine to cysteine

Locus: BSU_29310

Molecular weight: 49.25 kDa

Isoelectric point: 5.72

Protein length: 442 aa

Gene length: 1329 bp

Function: utilization of S-methyl-cysteine

Product: flavin-dependent monooxygenase

Essential: no

Synonyms: cmoJ, ytnJ

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2141 (Galperin et al., 2021)

Gene

Coordinates: 3,001,724  3,003,052

Phenotypes of a mutant

• no growth with S-methyl cysteine PubMed

The protein

Catalyzed reaction/ biological activity

• oxidation of S-methyl-cysteine PubMed
• conversion of N-acetyl-S-benzyl-l-cysteine sulfoxide to benzaldehyde and N-acetyl-l-cysteine sulfenic acid PubMed

Protein family

• ntaA/snaA/soxA(dszA) monooxygenase family (with YxeK, according to UniProt)

Cofactors

• FMN PubMed

Structure

• 1YW1 (PDB) (complex with FMN),  1TVL (PDB), 6ASK (PDB), 6ASL (PDB)
• O34974 (AlphaFold)

Paralogous protein(s)

• YxeK

Expression and Regulation

Operons

• Genes: snaA-tcyJ-tcyK-tcyL-tcyM-tcyN-cmoO-cmoI-cmoJ-ribR-sndA-ytnM

  Description: PubMed

  Regulation

  • induced in the presence of methionine and taurine PubMed

  Regulatory mechanism

  • CymR: repression, PubMed, in cymR regulon
  • AscR: activation, PubMed, in ascR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A155 (ytnJ::erm), available at the NBRP B. subtilis, Japan
• BKE29310 (cmoJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAACTGAATAAAATCTGCAC,  downstream forward: _UP4_TAGGTTTCGTCTGATCGAAT
• BKK29310 (cmoJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAACTGAATAAAATCTGCAC,  downstream forward: _UP4_TAGGTTTCGTCTGATCGAAT

References

Loading