Difference between revisions of "IleS"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
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=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' | + | * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1qu2 1QU2] (from ''Staphylococcus aureus'', 61% identity) {{PubMed|10446055}} |
* '''UniProt:''' [http://www.uniprot.org/uniprot/Q45477 Q45477] | * '''UniProt:''' [http://www.uniprot.org/uniprot/Q45477 Q45477] | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ileS_1613357_1616122_1 ileS] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ileS_1613357_1616122_1 ileS] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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=References= | =References= | ||
| − | <pubmed>19258532,, 17981983, 19233850 </pubmed> | + | <pubmed>19258532, 10446055, 17981983, 19233850 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 14:50, 12 July 2013
- Description: isoleucyl-tRNA synthetase
| Gene name | ileS |
| Synonyms | |
| Essential | yes PubMed |
| Product | isoleucyl-tRNA synthetase |
| Function | translation |
| Gene expression levels in SubtiExpress: ileS | |
| Metabolic function and regulation of this protein in SubtiPathways: tRNA charging | |
| MW, pI | 104 kDa, 5.191 |
| Gene length, protein length | 2763 bp, 921 aa |
| Immediate neighbours | divIVA, ylyA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU15430
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile) (according to Swiss-Prot)
- Protein family: IleS type 1 subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cytoplasm (according to Swiss-Prot)
Database entries
- UniProt: Q45477
- KEGG entry: [2]
- E.C. number: 6.1.1.5
Additional information
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and regulation
- Operon:
- Regulatory mechanism:
- T-box: RNA switch, transcriptional antitermination PubMed
- Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
