Difference between revisions of "UppS"
| Line 92: | Line 92: | ||
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' | + | * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=1X09 1X09] (the protein from ''E. coli'', 49% identity, 77% similarity) {{PubMed|15788389}} |
* '''UniProt:''' [http://www.uniprot.org/uniprot/O31751 O31751] | * '''UniProt:''' [http://www.uniprot.org/uniprot/O31751 O31751] | ||
| Line 135: | Line 135: | ||
=References= | =References= | ||
| − | <pubmed> 22904278 23796923 </pubmed> | + | <pubmed> 22904278 23796923 15788389 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:08, 1 July 2013
- Description: undecaprenyl pyrophosphate synthetase, provides the precursor for the carrier lipid, undecaprenyl phosphate (C55-P), which is required for cell wall synthesis
| Gene name | uppS |
| Synonyms | yluA |
| Essential | yes PubMed |
| Product | undecaprenyl pyrophosphate synthetase |
| Function | cell wall biosynthesis |
| Gene expression levels in SubtiExpress: uppS | |
| MW, pI | 29 kDa, 5.408 |
| Gene length, protein length | 780 bp, 260 aa |
| Immediate neighbours | frr, cdsA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell wall synthesis, biosynthesis of lipids, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16530
Phenotypes of a mutant
- essential PubMed
- the uppS86 (C257T) mutation provides resistance to rare earth metals such as scandium and to bacitracin PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: catalyzes eight consecutive condensation reactions of isopentenyl pyrophosphate (IPP) with farnesyl pyrophosphate (FPP) to form undecaprenyl pyrophosphate
- Protein family: UPP synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: O31751
- KEGG entry: [2]
- E.C. number: 2.5.1.31
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Yong Heon Lee, John D Helmann
Reducing the Level of Undecaprenyl Pyrophosphate Synthase Has Complex Effects on Susceptibility to Cell Wall Antibiotics.
Antimicrob Agents Chemother: 2013, 57(9);4267-4275
[PubMed:23796923]
[WorldCat.org]
[DOI]
(I p)
Takashi Inaoka, Kozo Ochi
Undecaprenyl pyrophosphate involvement in susceptibility of Bacillus subtilis to rare earth elements.
J Bacteriol: 2012, 194(20);5632-7
[PubMed:22904278]
[WorldCat.org]
[DOI]
(I p)
Rey-Ting Guo, Tzu-Ping Ko, Annie P-C Chen, Chih-Jung Kuo, Andrew H-J Wang, Po-Huang Liang
Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate, and farnesyl thiopyrophosphate: roles of the metal ion and conserved residues in catalysis.
J Biol Chem: 2005, 280(21);20762-74
[PubMed:15788389]
[WorldCat.org]
[DOI]
(P p)
