Difference between revisions of "PGP574"

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pGP574
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Vector for the expression of proteins in ''E. coli'', the [[plasmids|plasmid]] allows to fuse a Strep-tag to the C-terminus of the expressed protein
 
 
vector for the expression of proteins in ''E. coli'', the [[plasmids|plasmid]] allows to fuse a Strep-tag to the C-terminus of the expressed protein
 
  
 
host: ''E. coli'' BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
 
host: ''E. coli'' BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
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based on pET3C
 
based on pET3C
  
similar plasmid: [[pGP172]]
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similar [[plasmid]]: [[pGP172]]
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* sequencing primer:
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**'''T7:''' 5’-TAATACGACTCACTATAGGG -3’
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** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
  
Schilling, O., Herzberg, C., Hertrich, T., Vörsmann, H., Jessen, D., Hübner, S., Titgemeyer, F. & Stülke, J. (2006) Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples. Nucl. Acids Res. 34: 6102-6115. [http://www.ncbi.nlm.nih.gov/sites/entrez/17074746 PubMed]
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<pubmed> 17074746 </pubmed>
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==  Return to [[Plasmids]] ==

Latest revision as of 14:04, 3 June 2013

Vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the C-terminus of the expressed protein

host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

constructed in the Stülke lab

in E. coli: ampicillin resistance

based on pET3C

similar plasmid: pGP172

  • sequencing primer:
    • T7: 5’-TAATACGACTCACTATAGGG -3’
    • NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’

Return to Plasmids