Difference between revisions of "PGP172"

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* vector for the expression of proteins in E. coli, the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein
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* vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein
  
* host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
+
* host: ''E. coli'' BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
  
 
* constructed in the [[Stülke]] lab
 
* constructed in the [[Stülke]] lab
  
* in E. coli: ampicillin resistance
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* in ''E. coli'': ampicillin resistance
  
 
* based on pET3C
 
* based on pET3C
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**'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
 
**'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
 
** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
 
** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
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* important: ''Eco''RI, although mentioned in the MCS, is not unique!
  
 
<pubmed> 14717704 </pubmed>
 
<pubmed> 14717704 </pubmed>
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==  Return to [[Plasmids]] ==

Latest revision as of 14:04, 3 June 2013

pGP172: click to enlarge
pGP172 cloning region: click to enlarge


  • vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein
  • host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
  • in E. coli: ampicillin resistance
  • based on pET3C
  • sequencing primer:
    • CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
    • NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
  • important: EcoRI, although mentioned in the MCS, is not unique!

Return to Plasmids