DacB

• Description: sporulation-specific penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase
  
  

Gene name	dacB
Synonyms
Essential	no
Product	penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase
Function	carboxypeptidase
Gene expression levels in SubtiExpress: dacB
MW, pI	42 kDa, 9.725
Gene length, protein length	1146 bp, 382 aa
Immediate neighbours	spmA, ypuI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU23190

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: modifies degree of cross-linking of glycan strands in peptidoglycan

• Protein family: peptidase S11 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure: 3MFD (beta-lactamase superfamily domain)

• UniProt: P35150

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dacB-spmA-spmB PubMed

• Expression browser: dacB PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A744 ( dacB::cat), PubMed, available at BGSC
  • 1A745 ( dacB::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Pengfei Zhang, Stacy Thomas, Yong-qing Li, Peter Setlow
Effects of cortex peptidoglycan structure and cortex hydrolysis on the kinetics of Ca(2+)-dipicolinic acid release during Bacillus subtilis spore germination.
J Bacteriol: 2012, 194(3);646-52
[PubMed:22123250] [WorldCat.org] [DOI] (I p)

Ralf Moeller, Peter Setlow, Günther Reitz, Wayne L Nicholson
Roles of small, acid-soluble spore proteins and core water content in survival of Bacillus subtilis spores exposed to environmental solar UV radiation.
Appl Environ Microbiol: 2009, 75(16);5202-8
[PubMed:19542328] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Dirk-Jan Scheffers, Laura J F Jones, Jeffery Errington
Several distinct localization patterns for penicillin-binding proteins in Bacillus subtilis.
Mol Microbiol: 2004, 51(3);749-64
[PubMed:14731276] [WorldCat.org] [DOI] (P p)

D L Popham, J Meador-Parton, C E Costello, P Setlow
Spore peptidoglycan structure in a cwlD dacB double mutant of Bacillus subtilis.
J Bacteriol: 1999, 181(19);6205-9
[PubMed:10498740] [WorldCat.org] [DOI] (P p)

A Atrih, G Bacher, G Allmaier, M P Williamson, S J Foster
Analysis of peptidoglycan structure from vegetative cells of Bacillus subtilis 168 and role of PBP 5 in peptidoglycan maturation.
J Bacteriol: 1999, 181(13);3956-66
[PubMed:10383963] [WorldCat.org] [DOI] (P p)

D L Popham, M E Gilmore, P Setlow
Roles of low-molecular-weight penicillin-binding proteins in Bacillus subtilis spore peptidoglycan synthesis and spore properties.
J Bacteriol: 1999, 181(1);126-32
[PubMed:9864321] [WorldCat.org] [DOI] (P p)

D L Popham, B Illades-Aguiar, P Setlow
The Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is part of a three-gene operon required for proper spore cortex synthesis and spore core dehydration.
J Bacteriol: 1995, 177(16);4721-9
[PubMed:7642500] [WorldCat.org] [DOI] (P p)

E B Simpson, T W Hancock, C E Buchanan
Transcriptional control of dacB, which encodes a major sporulation-specific penicillin-binding protein.
J Bacteriol: 1994, 176(24);7767-9
[PubMed:7528199] [WorldCat.org] [DOI] (P p)

C E Buchanan, M L Ling
Isolation and sequence analysis of dacB, which encodes a sporulation-specific penicillin-binding protein in Bacillus subtilis.
J Bacteriol: 1992, 174(6);1717-25
[PubMed:1548223] [WorldCat.org] [DOI] (P p)