OdhA

• Description: 2-oxoglutarate dehydrogenase (E1 subunit)
  
  

Gene name	odhA
Synonyms	citK
Essential	no
Product	2-oxoglutarate dehydrogenase (E1 subunit)
Function	TCA cycle
Gene expression levels in SubtiExpress: odhA
Interactions involving this protein in SubtInteract: OdhA
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	105 kDa, 5.871
Gene length, protein length	2823 bp, 941 aa
Immediate neighbours	odhB, yojO
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

• Locus tag: BSU19370

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO₂ (according to Swiss-Prot)

• Protein family: 2-oxoacid dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-66 and Arg-621 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • OdhA-OdhB-PdhD PubMed

• Localization:

Database entries

• Structure: 2JGD (from Escherichia coli, 38% identity, 55% similarity) PubMed

• UniProt: P23129

• KEGG entry: [3]

• E.C. number: 1.2.4.2

Additional information

• extensive information on the structure and enzymatic properties of 2-oxoglutarate dehydrogenase can be found at Proteopedia

Expression and regulation

• Operon: odhA-odhB PubMed

• Expression browser: odhA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (2.4-fold) (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP671 (spc), GP684 (cat), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

References

Reviews

K F Sheu, J P Blass
The alpha-ketoglutarate dehydrogenase complex.
Ann N Y Acad Sci: 1999, 893;61-78
[PubMed:10672230] [WorldCat.org] [DOI] (P p)