PhrC

• Description: response regulator aspartate phosphatase (RapC) regulator / competence and sporulation stimulating factor (CSF)
  
  

Gene name	phrC
Synonyms
Essential	no
Product	phosphatase (RapC) regulator / competence and sporulation stimulating factor (CSF)
Function	control of ComA activity
Gene expression levels in SubtiExpress: phrC
Interactions involving this protein in SubtInteract: PhrC
MW, pI	4 kDa, 8.16
Gene length, protein length	120 bp, 40 aa
Immediate neighbours	rapC, yczM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, transcription factors and their control, quorum sensing, short peptides

This gene is a member of the following regulons

CcpA regulon, CodY regulon, ComA regulon, SigH regulon

The gene

Basic information

• Locus tag: BSU03780

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds to RapC, this results in the inability of RapC to interact with ComA PubMed

• Protein family: phr family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RapC-PhrC PubMed
  • RapJ-PhrC PubMed

• Localization:

Database entries

• Structure:
  • 4GYO (complex RapJ-PhrC) PubMed

• UniProt: P94416

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • rapC-phrC PubMed
  • phrC PubMed

• Expression browser: phrC PubMed

• Sigma factor:
  • rapC: SigA PubMed
  • phrC: SigH PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • repressed by glucose (4.5-fold) (CcpA) PubMed
  • expressed at high cell density (ComA) PubMed

• Regulatory mechanism:
  • ComA: transcription activation (ComA) PubMed
  • CodY: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Elizabeth Anne Shank, Roberto Kolter
Extracellular signaling and multicellularity in Bacillus subtilis.
Curr Opin Microbiol: 2011, 14(6);741-7
[PubMed:22024380] [WorldCat.org] [DOI] (I p)

Original publications

Vijay Parashar, Philip D Jeffrey, Matthew B Neiditch
Conformational change-induced repeat domain expansion regulates Rap phosphatase quorum-sensing signal receptors.
PLoS Biol: 2013, 11(3);e1001512
[PubMed:23526881] [WorldCat.org] [DOI] (I p)

Ju Jung, Kyung Ok Yu, Ahmad Bazli Ramzi, Se Hoon Choe, Seung Wook Kim, Sung Ok Han
Improvement of surfactin production in Bacillus subtilis using synthetic wastewater by overexpression of specific extracellular signaling peptides, comX and phrC.
Biotechnol Bioeng: 2012, 109(9);2349-56
[PubMed:22511326] [WorldCat.org] [DOI] (I p)

Kassem Hamze, Daria Julkowska, Sabine Autret, Krzysztof Hinc, Krzysztofa Nagorska, Agnieszka Sekowska, I Barry Holland, Simone J Séror
Identification of genes required for different stages of dendritic swarming in Bacillus subtilis, with a novel role for phrC.
Microbiology (Reading): 2009, 155(Pt 2);398-412
[PubMed:19202088] [WorldCat.org] [DOI] (P p)

Mridula Pottathil, April Jung, Beth A Lazazzera
CSF, a species-specific extracellular signaling peptide for communication among strains of Bacillus subtilis and Bacillus mojavensis.
J Bacteriol: 2008, 190(11);4095-9
[PubMed:18375560] [WorldCat.org] [DOI] (I p)

Jennifer M Auchtung, Catherine A Lee, Alan D Grossman
Modulation of the ComA-dependent quorum response in Bacillus subtilis by multiple Rap proteins and Phr peptides.
J Bacteriol: 2006, 188(14);5273-85
[PubMed:16816200] [WorldCat.org] [DOI] (P p)

Natalia Comella, Alan D Grossman
Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis.
Mol Microbiol: 2005, 57(4);1159-74
[PubMed:16091051] [WorldCat.org] [DOI] (P p)

Leighton Core, Marta Perego
TPR-mediated interaction of RapC with ComA inhibits response regulator-DNA binding for competence development in Bacillus subtilis.
Mol Microbiol: 2003, 49(6);1509-22
[PubMed:12950917] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

A Yazgan, G Ozcengiz, M A Marahiel
Tn10 insertional mutations of Bacillus subtilis that block the biosynthesis of bacilysin.
Biochim Biophys Acta: 2001, 1518(1-2);87-94
[PubMed:11267663] [WorldCat.org] [DOI] (P p)

B A Lazazzera, I G Kurtser, R S McQuade, A D Grossman
An autoregulatory circuit affecting peptide signaling in Bacillus subtilis.
J Bacteriol: 1999, 181(17);5193-200
[PubMed:10464187] [WorldCat.org] [DOI] (P p)