Difference between revisions of "CwlD"

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Revision as of 12:24, 16 May 2013

  • Description: N-acetylmuramoyl-L-alanine amidase

Gene name cwlD
Synonyms
Essential no
Product N-acetylmuramoyl-L-alanine amidase
Function spore germination
Gene expression levels in SubtiExpress: cwlD
MW, pI 26 kDa, 9.788
Gene length, protein length 711 bp, 237 aa
Immediate neighbours ybaK, salA
Sequences Protein DNA DNA_with_flanks
Genetic context
CwlD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CwlD expression.png
























Categories containing this gene/protein

germination

This gene is a member of the following regulons

LexA regulon, SigE regulon, SigG regulon

The gene

Basic information

  • Locus tag: BSU01530

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
    • formation of muramic acid delta-lacton in peptidoglycan
  • Protein family: N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290] [WorldCat.org] [DOI] (P p)

Meghan E Gilmore, Dabanjan Bandyopadhyay, Amanda M Dean, Sarah D Linnstaedt, David L Popham
Production of muramic delta-lactam in Bacillus subtilis spore peptidoglycan.
J Bacteriol: 2004, 186(1);80-9
[PubMed:14679227] [WorldCat.org] [DOI] (P p)

Ann E Cowan, Dennis E Koppel, Barbara Setlow, Peter Setlow
A soluble protein is immobile in dormant spores of Bacillus subtilis but is mobile in germinated spores: implications for spore dormancy.
Proc Natl Acad Sci U S A: 2003, 100(7);4209-14
[PubMed:12646705] [WorldCat.org] [DOI] (P p)

D L Popham, J Meador-Parton, C E Costello, P Setlow
Spore peptidoglycan structure in a cwlD dacB double mutant of Bacillus subtilis.
J Bacteriol: 1999, 181(19);6205-9
[PubMed:10498740] [WorldCat.org] [DOI] (P p)

J Sekiguchi, K Akeo, H Yamamoto, F K Khasanov, J C Alonso, A Kuroda
Nucleotide sequence and regulation of a new putative cell wall hydrolase gene, cwlD, which affects germination in Bacillus subtilis.
J Bacteriol: 1995, 177(19);5582-9
[PubMed:7559346] [WorldCat.org] [DOI] (P p)