Difference between revisions of "YabT"
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Revision as of 12:19, 16 May 2013
- Description: Ser/Thr kinase, controls DNA integrity during spore development
| Gene name | yabT |
| Synonyms | |
| Essential | no |
| Product | Ser/Thr kinase |
| Function | control of DNA integrity during spore development |
| Gene expression levels in SubtiExpress: yabT | |
| Interactions involving this protein in SubtInteract: YabT | |
| MW, pI | 37 kDa, 9.943 |
| Gene length, protein length | 1014 bp, 338 aa |
| Immediate neighbours | yabS, tilS |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
protein modification, sporulation proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00660
Phenotypes of a mutant
- increased sensitivity to DNA damage during spore development PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: protein kinase domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- autophosphorylation is stimulated by non-specific binding to DNA PubMed
- Localization:
- colocalizes strongly with the septal membrane separating the mother cells from the forespore PubMed
Database entries
- Structure:
- UniProt: P37562
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP577 (erm), available in Jörg Stülke's lab
- Expression vector:
- purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP391, available in Jörg Stülke's lab
- purification from E. coli with N-terminal Strep-tag, in pGP172: pGP823, available in Jörg Stülke's lab
- purification from E. coli with N-terminal His-tag, in pWH844: pGP1408, available in Jörg Stülke's lab
- lacZ fusion:
- translational lacZ fusion (in pAC7): pGP831, available in Jörg Stülke's lab
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Vladimir Bidnenko, Lei Shi, Ahasanul Kobir, Magali Ventroux, Nathalie Pigeonneau, Céline Henry, Alain Trubuil, Marie-Françoise Noirot-Gros, Ivan Mijakovic
Bacillus subtilis serine/threonine protein kinase YabT is involved in spore development via phosphorylation of a bacterial recombinase.
Mol Microbiol: 2013, 88(5);921-35
[PubMed:23634894]
[WorldCat.org]
[DOI]
(I p)
Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325]
[WorldCat.org]
[DOI]
(P p)
