BmrA

• Description: multidrug ABC transporter (ATP-binding protein)
  
  

Gene name	bmrA
Synonyms	yvcC
Essential	no
Product	multidrug ABC transporter (ATP-binding protein)
Function	multiple antibiotic resistance
Gene expression levels in SubtiExpress: bmrA
MW, pI	64 kDa, 6.474
Gene length, protein length	1767 bp, 589 aa
Immediate neighbours	yvcD, yvzA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, resistance against toxins/ antibiotics, membrane proteins

The gene

Basic information

• Locus tag: BSU34820

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: has both a membrane-spanning and an ATP-binding domain PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane PubMed

Database entries

• Structure:

• UniProt: O06967

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: bmrA PubMed

• Sigma factor: SigA PubMed

• Regulation: constitutively expressed PubMed

• Regulatory mechanism:

• Additional information:
  • half-life of the mRNA: 1.5 min PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Kerry J Lee, Lauren M Browning, Tao Huang, Feng Ding, Prakash D Nallathamby, Xiao-Hong Nancy Xu
Probing of multidrug ABC membrane transporters of single living cells using single plasmonic nanoparticle optical probes.
Anal Bioanal Chem: 2010, 397(8);3317-28
[PubMed:20544182] [WorldCat.org] [DOI] (I p)

Cédric Orelle, Francesca Gubellini, Anne Durand, Sergio Marco, Daniel Lévy, Philippe Gros, Attilio Di Pietro, Jean-Michel Jault
Conformational change induced by ATP binding in the multidrug ATP-binding cassette transporter BmrA.
Biochemistry: 2008, 47(8);2404-12
[PubMed:18215075] [WorldCat.org] [DOI] (P p)

Stéphanie Ravaud, Marie-Ange Do Cao, Marie Jidenko, Christine Ebel, Marc Le Maire, Jean-Michel Jault, Attilio Di Pietro, Richard Haser, Nushin Aghajari
The ABC transporter BmrA from Bacillus subtilis is a functional dimer when in a detergent-solubilized state.
Biochem J: 2006, 395(2);345-53
[PubMed:16405427] [WorldCat.org] [DOI] (I p)

Olivier Dalmas, Cédric Orelle, Anne-Emmanuelle Foucher, Christophe Geourjon, Serge Crouzy, Attilio Di Pietro, Jean-Michel Jault
The Q-loop disengages from the first intracellular loop during the catalytic cycle of the multidrug ABC transporter BmrA.
J Biol Chem: 2005, 280(44);36857-64
[PubMed:16107340] [WorldCat.org] [DOI] (P p)

Olivier Dalmas, Marie-Ange Do Cao, Miguel R Lugo, Frances J Sharom, Attilio Di Pietro, Jean-Michel Jault
Time-resolved fluorescence resonance energy transfer shows that the bacterial multidrug ABC half-transporter BmrA functions as a homodimer.
Biochemistry: 2005, 44(11);4312-21
[PubMed:15766260] [WorldCat.org] [DOI] (P p)

Emmanuelle Steinfels, Cédric Orelle, Jean-Raphaël Fantino, Olivier Dalmas, Jean-Louis Rigaud, François Denizot, Attilio Di Pietro, Jean-Michel Jault
Characterization of YvcC (BmrA), a multidrug ABC transporter constitutively expressed in Bacillus subtilis.
Biochemistry: 2004, 43(23);7491-502
[PubMed:15182191] [WorldCat.org] [DOI] (P p)

Cédric Orelle, Olivier Dalmas, Philippe Gros, Attilio Di Pietro, Jean-Michel Jault
The conserved glutamate residue adjacent to the Walker-B motif is the catalytic base for ATP hydrolysis in the ATP-binding cassette transporter BmrA.
J Biol Chem: 2003, 278(47);47002-8
[PubMed:12968023] [WorldCat.org] [DOI] (P p)

Emmanuelle Steinfels, Cédric Orelle, Olivier Dalmas, François Penin, Bruno Miroux, Attilio Di Pietro, Jean-Michel Jault
Highly efficient over-production in E. coli of YvcC, a multidrug-like ATP-binding cassette transporter from Bacillus subtilis.
Biochim Biophys Acta: 2002, 1565(1);1-5
[PubMed:12225846] [WorldCat.org] [DOI] (P p)

Mohamed Chami, Emmanuelle Steinfels, Cédric Orelle, Jean-Michel Jault, Attilio Di Pietro, Jean-Louis Rigaud, Sergio Marco
Three-dimensional structure by cryo-electron microscopy of YvcC, an homodimeric ATP-binding cassette transporter from Bacillus subtilis.
J Mol Biol: 2002, 315(5);1075-85
[PubMed:11827477] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)