Difference between revisions of "LeuS"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytwF]]'', ''[[ytvB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytwF]]'', ''[[ytvB]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU30320 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU30320 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU30320 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU30320 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU30320 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU30320 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:leuS_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:leuS_context.gif]]

Revision as of 11:04, 14 May 2013

  • Description: Leu-tRNA synthetase

Gene name leuS
Synonyms
Essential yes PubMed
Product leucyl-tRNA synthetase
Function translation
Gene expression levels in SubtiExpress: leuS
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 91 kDa, 4.857
Gene length, protein length 2412 bp, 804 aa
Immediate neighbours ytwF, ytvB
Sequences Protein DNA DNA_with_flanks
Genetic context
LeuS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LeuS expression.png




























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU30320

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu) (according to Swiss-Prot)
  • Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2AJG (from Escherichia coli, 39% identity, 56% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Original Publications

P B Vander Horn, S A Zahler
Cloning and nucleotide sequence of the leucyl-tRNA synthetase gene of Bacillus subtilis.
J Bacteriol: 1992, 174(12);3928-35
[PubMed:1317842] [WorldCat.org] [DOI] (P p)