Difference between revisions of "RecN"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoIVB]]'', ''[[ahrC]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoIVB]]'', ''[[ahrC]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU24240 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU24240 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU24240 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU24240 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU24240 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU24240 DNA_with_flanks]
 
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Revision as of 10:41, 14 May 2013

  • Description: DNA repair and genetic recombination

Gene name recN
Synonyms
Essential no
Product unknown
Function DNA repair and genetic recombination
Gene expression levels in SubtiExpress: recN
MW, pI 64 kDa, 4.802
Gene length, protein length 1728 bp, 576 aa
Immediate neighbours spoIVB, ahrC
Sequences Protein DNA DNA_with_flanks
Genetic context
RecN context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RecN expression.png















Categories containing this gene/protein

DNA repair/ recombination, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU24240

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • RecN stimulates polymerizing activity of PnpA PubMed
  • Protein family: recN family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

Your additional remarks

References

Reviews

Additional reviews: PubMed

Silvia Ayora, Begoña Carrasco, Paula P Cárdenas, Carolina E César, Cristina Cañas, Tribhuwan Yadav, Chiara Marchisone, Juan C Alonso
Double-strand break repair in bacteria: a view from Bacillus subtilis.
FEMS Microbiol Rev: 2011, 35(6);1055-81
[PubMed:21517913] [WorldCat.org] [DOI] (I p)

Peter L Graumann, Tobias Knust
Dynamics of the bacterial SMC complex and SMC-like proteins involved in DNA repair.
Chromosome Res: 2009, 17(2);265-75
[PubMed:19308706] [WorldCat.org] [DOI] (I p)


Original publications

Additional publications: PubMed

Dawit Kidane, Begoña Carrasco, Candela Manfredi, Katharina Rothmaier, Silvia Ayora, Serkalem Tadesse, Juan C Alonso, Peter L Graumann
Evidence for different pathways during horizontal gene transfer in competent Bacillus subtilis cells.
PLoS Genet: 2009, 5(9);e1000630
[PubMed:19730681] [WorldCat.org] [DOI] (I p)

Lyle A Simmons, Alexi I Goranov, Hajime Kobayashi, Bryan W Davies, Daniel S Yuan, Alan D Grossman, Graham C Walker
Comparison of responses to double-strand breaks between Escherichia coli and Bacillus subtilis reveals different requirements for SOS induction.
J Bacteriol: 2009, 191(4);1152-61
[PubMed:19060143] [WorldCat.org] [DOI] (I p)

Humberto Sanchez, Paula P Cardenas, Shige H Yoshimura, Kunio Takeyasu, Juan C Alonso
Dynamic structures of Bacillus subtilis RecN-DNA complexes.
Nucleic Acids Res: 2008, 36(1);110-20
[PubMed:17999999] [WorldCat.org] [DOI] (I p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Humberto Sanchez, Dawit Kidane, M Castillo Cozar, Peter L Graumann, Juan C Alonso
Recruitment of Bacillus subtilis RecN to DNA double-strand breaks in the absence of DNA end processing.
J Bacteriol: 2006, 188(2);353-60
[PubMed:16385024] [WorldCat.org] [DOI] (P p)

Humberto Sanchez, Juan C Alonso
Bacillus subtilis RecN binds and protects 3'-single-stranded DNA extensions in the presence of ATP.
Nucleic Acids Res: 2005, 33(7);2343-50
[PubMed:15849320] [WorldCat.org] [DOI] (I e)

B E Van Hoy, J A Hoch
Characterization of the spoIVB and recN loci of Bacillus subtilis.
J Bacteriol: 1990, 172(3);1306-11
[PubMed:2106508] [WorldCat.org] [DOI] (P p)