Difference between revisions of "LcfB"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhfK]]'', ''[[yhfM]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhfK]]'', ''[[yhfM]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10270 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10270 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10270 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10270 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10270 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10270 DNA_with_flanks]
 
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Revision as of 09:50, 14 May 2013

  • Description: long-chain fatty-acid-CoA ligase, involved in surfactin production

Gene name lcfB
Synonyms yhfL
Essential no
Product long-chain fatty-acid-CoA ligase
Function fatty acid degradation
Gene expression levels in SubtiExpress: lcfB
Metabolic function and regulation of this protein in SubtiPathways:
Fatty acid degradation
MW, pI 56 kDa, 5.301
Gene length, protein length 1539 bp, 513 aa
Immediate neighbours yhfK, yhfM
Sequences Protein DNA DNA_with_flanks
Genetic context
YhfL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LcfB expression.png




























Categories containing this gene/protein

utilization of lipids

This gene is a member of the following regulons

CcpA regulon, FadR regulon

The gene

Basic information

  • Locus tag: BSU10270

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis PubMed
  • Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Femke I Kraas, Verena Helmetag, Melanie Wittmann, Matthias Strieker, Mohamed A Marahiel
Functional dissection of surfactin synthetase initiation module reveals insights into the mechanism of lipoinitiation.
Chem Biol: 2010, 17(8);872-80
[PubMed:20797616] [WorldCat.org] [DOI] (I p)

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)