Difference between revisions of "TcyP"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhcK]]'', ''[[yhcM]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhcK]]'', ''[[yhcM]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU09130 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU09130 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU09130 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU09130 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU09130 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU09130 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yhcL_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yhcL_context.gif]]

Revision as of 09:46, 14 May 2013

  • Description: cystine transporter, membrane protein

Gene name tcyP
Synonyms yhcL
Essential no
Product cystine transporter, membrane protein
Function cystine uptake
Gene expression levels in SubtiExpress: tcyP
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 48 kDa, 9.471
Gene length, protein length 1389 bp, 463 aa
Immediate neighbours yhcK, yhcM
Sequences Protein DNA DNA_with_flanks
Genetic context
YhcL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TcyP expression.png




























Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids, membrane proteins

This gene is a member of the following regulons

CymR regulon

The gene

Basic information

  • Locus tag: BSU09130

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: cystine-proton symporter PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: K(m) = 0.6 myM PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: tcyP
  • Sigma factor:
  • Regulation:
    • repressed in the presence of sulfate or cysteine (CymR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France

Your additional remarks

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Sergine Even, Pierre Burguière, Sandrine Auger, Olga Soutourina, Antoine Danchin, Isabelle Martin-Verstraete
Global control of cysteine metabolism by CymR in Bacillus subtilis.
J Bacteriol: 2006, 188(6);2184-97
[PubMed:16513748] [WorldCat.org] [DOI] (P p)

Pierre Burguière, Sandrine Auger, Marie-Françoise Hullo, Antoine Danchin, Isabelle Martin-Verstraete
Three different systems participate in L-cystine uptake in Bacillus subtilis.
J Bacteriol: 2004, 186(15);4875-84
[PubMed:15262924] [WorldCat.org] [DOI] (P p)

Sandrine Auger, Antoine Danchin, Isabelle Martin-Verstraete
Global expression profile of Bacillus subtilis grown in the presence of sulfate or methionine.
J Bacteriol: 2002, 184(18);5179-86
[PubMed:12193636] [WorldCat.org] [DOI] (P p)