Difference between revisions of "TsaB"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tsaE]]'', ''[[ydiD]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tsaE]]'', ''[[ydiD]]''
 
|-
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU05920 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU05920 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU05920 Advanced_DNA]
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU05920 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU05920 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU05920 DNA_with_flanks]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ydiC_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ydiC_context.gif]]

Revision as of 09:35, 14 May 2013

  • Description: required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA

Gene name tsaB
Synonyms ydiC
Essential yes PubMed
Product tRNA modification enzyme
Function tRNA modification
Gene expression levels in SubtiExpress: tsaB
Interactions involving this protein in SubtInteract: TsaB
MW, pI 25 kDa, 5.762
Gene length, protein length 687 bp, 229 aa
Immediate neighbours tsaE, ydiD
Sequences Protein DNA DNA_with_flanks
Genetic context
YdiC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YdiC expression.png















Categories containing this gene/protein

essential genes, translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU05920

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP (TsaB-TsaD-TsaE) PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Charles T Lauhon
Mechanism of N6-threonylcarbamoyladenonsine (t(6)A) biosynthesis: isolation and characterization of the intermediate threonylcarbamoyl-AMP.
Biochemistry: 2012, 51(44);8950-63
[PubMed:23072323] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Basma El Yacoubi, Isabelle Hatin, Christopher Deutsch, Tamer Kahveci, Jean-Pierre Rousset, Dirk Iwata-Reuyl, Alexey G Murzin, Valérie de Crécy-Lagard
A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification.
EMBO J: 2011, 30(5);882-93
[PubMed:21285948] [WorldCat.org] [DOI] (I p)

Jennifer I Handford, Bérengère Ize, Grant Buchanan, Gareth P Butland, Jack Greenblatt, Andrew Emili, Tracy Palmer
Conserved network of proteins essential for bacterial viability.
J Bacteriol: 2009, 191(15);4732-49
[PubMed:19376873] [WorldCat.org] [DOI] (I p)

Alison Hunt, Joy P Rawlins, Helena B Thomaides, Jeff Errington
Functional analysis of 11 putative essential genes in Bacillus subtilis.
Microbiology (Reading): 2006, 152(Pt 10);2895-2907
[PubMed:17005971] [WorldCat.org] [DOI] (P p)