YcbK

• Description: similar to efflux system
  
  

Gene name	ycbK
Synonyms
Essential	no
Product	unknown
Function	unknown
Gene expression levels in SubtiExpress: ycbK
Metabolic function and regulation of this protein in SubtiPathways: Phe, Tyr, Trp
MW, pI	33 kDa, 9.572
Gene length, protein length	936 bp, 312 aa
Immediate neighbours	rtpA, ycbL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, membrane proteins

This gene is a member of the following regulons

T-box, TRAP regulon

The gene

Basic information

• Locus tag: BSU02540

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: DUF6 domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P42243

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: rtpA-ycbK PubMed

• Expression browser: ycbK PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • rtpA: induced by tryptophan limitation (T-box in the mRNA leader) PubMed
  • ycbK: induced by tryptophan limitation (MtrB in the mRNA leader) PubMed

• Regulatory mechanism:
  • T-box: transcription termination/ antitermination PubMed
  • MtrB: control of translation initiation (access of the ribosome to the Shine-Dalgarno sequence) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Helen Yakhnin, Alexander V Yakhnin, Paul Babitzke
The trp RNA-binding attenuation protein (TRAP) of Bacillus subtilis regulates translation initiation of ycbK, a gene encoding a putative efflux protein, by blocking ribosome binding.
Mol Microbiol: 2006, 61(5);1252-66
[PubMed:16879415] [WorldCat.org] [DOI] (P p)

J P Sarsero, E Merino, C Yanofsky
A Bacillus subtilis operon containing genes of unknown function senses tRNATrp charging and regulates expression of the genes of tryptophan biosynthesis.
Proc Natl Acad Sci U S A: 2000, 97(6);2656-61
[PubMed:10706627] [WorldCat.org] [DOI] (P p)