Difference between revisions of "YbgE"

From SubtiWiki
Jump to: navigation, search
Line 24: Line 24:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ybgB]]'', ''[[ybgF]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ybgB]]'', ''[[ybgF]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU02390 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU02390 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU02390 Advanced_DNA]
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU02390 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU02390 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU02390 DNA_with_flanks]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ybgE_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ybgE_context.gif]]

Revision as of 09:23, 14 May 2013

  • Description: branched-chain amino acid aminotransferase

Gene name ybgE
Synonyms
Essential no
Product branched-chain amino acid aminotransferase
Function biosynthesis of branched-chain amino acids
Gene expression levels in SubtiExpress: ybgE
Metabolic function and regulation of this protein in SubtiPathways:
Lipid synthesis, Ile, Leu, Val, Coenzyme A
MW, pI 39 kDa, 4.952
Gene length, protein length 1068 bp, 356 aa
Immediate neighbours ybgB, ybgF
Sequences Protein DNA DNA_with_flanks
Genetic context
YbgE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YbgE expression.png
































Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

  • Locus tag: BSU02390

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: l-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate (according to Swiss-Prot)
  • Protein family: class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
  • Paralogous protein(s): YwaA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed by casamino acids PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion: pGP248 (in pAC5), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References