Difference between revisions of "YbfK"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ybfJ]]'', ''[[pssA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ybfJ]]'', ''[[pssA]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU02260 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU02260 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU02260 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU02260 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU02260 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU02260 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:ybfK_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ybfK_context.gif]]

Revision as of 09:22, 14 May 2013

  • Description: carboxylesterase NP

Gene name ybfK
Synonyms cesB
Essential no
Product carboxylesterase NP
Function degradation of isoprenoid-containing lipids
Gene expression levels in SubtiExpress: ybfK
MW, pI 32 kDa, 5.135
Gene length, protein length 888 bp, 296 aa
Immediate neighbours ybfJ, pssA
Sequences Protein DNA DNA_with_flanks
Genetic context
YbfK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YbfK expression.png
























Categories containing this gene/protein

utilization of lipids

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU02260

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: A carboxylic ester + H2O = an alcohol + a carboxylate (according to Swiss-Prot)
  • Protein family: AB hydrolase superfamily (according to Swiss-Prot)
  • Paralogous protein(s): Nap

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):

Database entries

  • Structure: 2R11 (the structure of Nap, 60% identity, 87% similarity)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

M J Dröge, R Bos, W J Quax
Paralogous gene analysis reveals a highly enantioselective 1,2-O-isopropylideneglycerol caprylate esterase of Bacillus subtilis.
Eur J Biochem: 2001, 268(11);3332-8
[PubMed:11389736] [WorldCat.org] [DOI] (P p)