Difference between revisions of "DeaD"
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|style="background:#ABCDEF;" align="center"|'''Function''' || RNA helicase | |style="background:#ABCDEF;" align="center"|'''Function''' || RNA helicase | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39110 deaD] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/DeaD DeaD] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/DeaD DeaD] | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxiO]]'', ''[[yxiM]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxiO]]'', ''[[yxiM]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU39110 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU39110 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU39110 Advanced_DNA] |
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Revision as of 14:14, 13 May 2013
- Description: DEAD-box RNA helicase, important for adaptation to low temperatures
| Gene name | deaD |
| Synonyms | yxiN |
| Essential | no |
| Product | DEAD-box RNA helicase |
| Function | RNA helicase |
| Gene expression levels in SubtiExpress: deaD | |
| Interactions involving this protein in SubtInteract: DeaD | |
| MW, pI | 53 kDa, 7.706 |
| Gene length, protein length | 1437 bp, 479 aa |
| Immediate neighbours | yxiO, yxiM |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
DEAD-box RNA helicases, translation
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU39110
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Protein family: DEAD-box RNA helicase
Extended information on the protein
- Kinetic information:
- Domains:
- Helicase domain 1: AS 1-203
- Linker region: AS 204-212
- Helicase domain 2: AS 207-368
- RNA-binding domain: AS 404-479
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 2G0C (RNA-binding domain, AA 404-479), 2HJV (second domain, AA 207-368); 3MOJ (RNA binding domain complexed with a fragment of 23S ribosomal RNA) PubMed
- UniProt: P42305
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- expression declines in the stationary phase PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP1052 (tet), available in the Stülke lab
- Expression vector:
- for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1065 (spc), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- FLAG-tag construct:
- GP1068 (spc, based on pGP1331), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Dagmar Klostermeier, Biozentrum Basel, Switzerland homepage
Your additional remarks
References
