Difference between revisions of "SpsK"

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|style="background:#ABCDEF;" align="center"|'''Function''' || rhamnose biosynthesis, spore coat polysaccharide synthesis
 
|style="background:#ABCDEF;" align="center"|'''Function''' || rhamnose biosynthesis, spore coat polysaccharide synthesis
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU37820 spsK]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37820 spsK]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 6.081   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 6.081   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spsL]]'', ''[[spsJ]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spsL]]'', ''[[spsJ]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15808&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37820 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37820 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37820 Advanced_DNA]
 
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Revision as of 14:08, 13 May 2013

  • Description: dTDP -4-dehydrorhamnose reductase, spore coat polysaccharide synthesis

Gene name spsK
Synonyms ipa-73d
Essential no
Product dTDP -4-dehydrorhamnose reductase
Function rhamnose biosynthesis, spore coat polysaccharide synthesis
Gene expression levels in SubtiExpress: spsK
MW, pI 31 kDa, 6.081
Gene length, protein length 849 bp, 283 aa
Immediate neighbours spsL, spsJ
Sequences Protein DNA Advanced_DNA
Genetic context
SpsK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpsK expression.png















Categories containing this gene/protein

miscellaneous metabolic pathways, sporulation proteins

This gene is a member of the following regulons

SigE regulon, SigK regulon

The gene

Basic information

  • Locus tag: BSU37820

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: dTDP-4-dehydrorhamnose reductase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3SC6 (dTDP-4-dehydrorhamnose reductase (rfbD) from Bacillus anthracis str. Ames in complex with NADP; 42% identity, 72% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the mother cell (SigK) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Germán Plata, Tobias Fuhrer, Tzu-Lin Hsiao, Uwe Sauer, Dennis Vitkup
Global probabilistic annotation of metabolic networks enables enzyme discovery.
Nat Chem Biol: 2012, 8(10);848-54
[PubMed:22960854] [WorldCat.org] [DOI] (I p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933] [WorldCat.org] [DOI] (P p)