Difference between revisions of "LytD"

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|style="background:#ABCDEF;" align="center"|'''Function''' || major autolysin, cell separation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || major autolysin, cell separation
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU35780 lytD]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35780 lytD]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 95 kDa, 9.935   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 95 kDa, 9.935   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tagC]]'', ''[[pmi]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tagC]]'', ''[[pmi]]''
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15595&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35780 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35780 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35780 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:lytD_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:lytD_context.gif]]

Revision as of 14:00, 13 May 2013

  • Description: glucosaminidase

Gene name lytD
Synonyms cwlG
Essential no
Product glucosaminidase
Function major autolysin, cell separation
Gene expression levels in SubtiExpress: lytD
MW, pI 95 kDa, 9.935
Gene length, protein length 2640 bp, 880 aa
Immediate neighbours tagC, pmi
Sequences Protein DNA Advanced_DNA
Genetic context
LytD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LytD expression.png
























Categories containing this gene/protein

cell wall degradation/ turnover

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

  • Locus tag: BSU35780

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relexed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 73 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

Rui Chen, Sarah B Guttenplan, Kris M Blair, Daniel B Kearns
Role of the sigmaD-dependent autolysins in Bacillus subtilis population heterogeneity.
J Bacteriol: 2009, 191(18);5775-84
[PubMed:19542270] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

P Margot, C Mauël, D Karamata
The gene of the N-acetylglucosaminidase, a Bacillus subtilis 168 cell wall hydrolase not involved in vegetative cell autolysis.
Mol Microbiol: 1994, 12(4);535-45
[PubMed:7934877] [WorldCat.org] [DOI] (P p)