Difference between revisions of "SmpB"

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|style="background:#ABCDEF;" align="center"|'''Function''' || trans-translation  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || trans-translation  
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU33600 smpB]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33600 smpB]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 10.421   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 10.421   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ssrA]]'', ''[[rnr]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ssrA]]'', ''[[rnr]]''
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15365&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33600 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33600 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33600 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:smpB_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:smpB_context.gif]]

Revision as of 13:51, 13 May 2013

  • Description: tmRNA-binding protein

Gene name smpB
Synonyms yvaI
Essential no
Product tmRNA-binding protein
Function trans-translation
Gene expression levels in SubtiExpress: smpB
MW, pI 17 kDa, 10.421
Gene length, protein length 468 bp, 156 aa
Immediate neighbours ssrA, rnr
Sequences Protein DNA Advanced_DNA
Genetic context
SmpB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SmpB expression.png
























Categories containing this gene/protein

translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU33600

Phenotypes of a mutant

  • impaired growth at high and low temperatures PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: smpB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hiromi Ujiie, Tomoko Matsutani, Hisashi Tomatsu, Ai Fujihara, Chisato Ushida, Yasuhiko Miwa, Yasutaro Fujita, Hyouta Himeno, Akira Muto
Trans-translation is involved in the CcpA-dependent tagging and degradation of TreP in Bacillus subtilis.
J Biochem: 2009, 145(1);59-66
[PubMed:18977770] [WorldCat.org] [DOI] (I p)

Ji-Hyun Shin, Chester W Price
The SsrA-SmpB ribosome rescue system is important for growth of Bacillus subtilis at low and high temperatures.
J Bacteriol: 2007, 189(10);3729-37
[PubMed:17369301] [WorldCat.org] [DOI] (P p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Jeffrey H Withey, David I Friedman
A salvage pathway for protein structures: tmRNA and trans-translation.
Annu Rev Microbiol: 2003, 57;101-23
[PubMed:12730326] [WorldCat.org] [DOI] (P p)

T Wiegert, W Schumann
SsrA-mediated tagging in Bacillus subtilis.
J Bacteriol: 2001, 183(13);3885-9
[PubMed:11395451] [WorldCat.org] [DOI] (P p)

A W Karzai, E D Roche, R T Sauer
The SsrA-SmpB system for protein tagging, directed degradation and ribosome rescue.
Nat Struct Biol: 2000, 7(6);449-55
[PubMed:10881189] [WorldCat.org] [DOI] (P p)