Difference between revisions of "Tgl"
| Line 14: | Line 14: | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || introduction of crosslinks in the spore coat protein [[GerQ]] | |style="background:#ABCDEF;" align="center"|'''Function''' || introduction of crosslinks in the spore coat protein [[GerQ]] | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31270 tgl] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 6.687 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 6.687 | ||
| Line 22: | Line 22: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mcpB]]'', ''[[yuzH]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mcpB]]'', ''[[yuzH]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31270 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31270 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31270 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:tgl_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:tgl_context.gif]] | ||
Revision as of 13:42, 13 May 2013
- Description: spore associated transglutaminase
| Gene name | tgl |
| Synonyms | yuxF, yugV |
| Essential | no |
| Product | spore associated transglutaminase |
| Function | introduction of crosslinks in the spore coat protein GerQ |
| Gene expression levels in SubtiExpress: tgl | |
| MW, pI | 28 kDa, 6.687 |
| Gene length, protein length | 735 bp, 245 aa |
| Immediate neighbours | mcpB, yuzH |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU31270
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3 (according to Swiss-Prot), temperature-dependent modification of the coat proteins such as GerQ (together with YabG) PubMed
- Protein family: bacillus TGase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P40746
- KEGG entry: [3]
- E.C. number: 2.3.2.13
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Adriano Henriques, Lisbon, Portugal homepage
Your additional remarks
References
Reviews
Ritsuko Kuwana
[Protein modification system in dormant cells].
Yakugaku Zasshi: 2009, 129(10);1221-5
[PubMed:19797877]
[WorldCat.org]
[DOI]
(P p)
Original Publications
Diana Plácido, Catarina G Fernandes, Anabela Isidro, Maria Arménia Carrondo, Adriano O Henriques, Margarida Archer
Auto-induction and purification of a Bacillus subtilis transglutaminase (Tgl) and its preliminary crystallographic characterization.
Protein Expr Purif: 2008, 59(1);1-8
[PubMed:18249137]
[WorldCat.org]
[DOI]
(I p)
Ritsuko Kuwana, Naoyuki Okuda, Hiromu Takamatsu, Kazuhito Watabe
Modification of GerQ reveals a functional relationship between Tgl and YabG in the coat of Bacillus subtilis spores.
J Biochem: 2006, 139(5);887-901
[PubMed:16751597]
[WorldCat.org]
[DOI]
(P p)
Rita Zilhão, Rachele Isticato, Lígia O Martins, Leif Steil, Uwe Völker, Ezio Ricca, Charles P Moran, Adriano O Henriques
Assembly and function of a spore coat-associated transglutaminase of Bacillus subtilis.
J Bacteriol: 2005, 187(22);7753-64
[PubMed:16267299]
[WorldCat.org]
[DOI]
(P p)
Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190]
[WorldCat.org]
[DOI]
(P p)
Katsunori Kobayashi, Shun-Ichi Suzuki, Yuko Izawa, Kiyoshi Miwa, Shigeru Yamanaka
Transglutaminase in sporulating cells of Bacillus subtilis.
J Gen Appl Microbiol: 1998, 44(1);85-91
[PubMed:12501297]
[WorldCat.org]
[DOI]
(I p)
S Suzuki, Y Izawa, K Kobayashi, Y Eto, S Yamanaka, K Kubota, K Yokozeki
Purification and characterization of novel transglutaminase from Bacillus subtilis spores.
Biosci Biotechnol Biochem: 2000, 64(11);2344-51
[PubMed:11193401]
[WorldCat.org]
[DOI]
(P p)
Bauke Oudega, Gregory Koningstein, Luísa Rodrigues, Maria de Sales Ramon, Helmut Hilbert, Andreas Düsterhöft, Thomas M Pohl, Thomas Weitzenegger
Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai).
Microbiology (Reading): 1997, 143 ( Pt 8);2769-2774
[PubMed:9274030]
[WorldCat.org]
[DOI]
(P p)
