Difference between revisions of "AmyX"
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|style="background:#ABCDEF;" align="center"|'''Function''' || starch degradation | |style="background:#ABCDEF;" align="center"|'''Function''' || starch degradation | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29930 amyX] |
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 80 kDa, 5.799 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 80 kDa, 5.799 | ||
| Line 22: | Line 22: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytmP]]'', ''[[ytlR]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytmP]]'', ''[[ytlR]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29930 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29930 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29930 Advanced_DNA] |
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Revision as of 13:38, 13 May 2013
- Description: pullulanase (debranching enzyme)
| Gene name | amyX |
| Synonyms | |
| Essential | no |
| Product | pullulanase |
| Function | starch degradation |
| Gene expression levels in SubtiExpress: amyX | |
| MW, pI | 80 kDa, 5.799 |
| Gene length, protein length | 2154 bp, 718 aa |
| Immediate neighbours | ytmP, ytlR |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
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Contents
Categories containing this gene/protein
utilization of specific carbon sources
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29930
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): GlgB
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: C0SPA0
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Jae-Hoon Shim, Jong-Tae Park, Jung-Sun Hong, Ki Woo Kim, Myo-Jeong Kim, Jung-Hyuk Auh, Young-Wan Kim, Cheon-Seok Park, Winfried Boos, Jung-Wan Kim, Kwan-Hwa Park
Role of maltogenic amylase and pullulanase in maltodextrin and glycogen metabolism of Bacillus subtilis 168.
J Bacteriol: 2009, 191(15);4835-44
[PubMed:19465663]
[WorldCat.org]
[DOI]
(I p)
Dominggus Malle, Takafumi Itoh, Wataru Hashimoto, Kousaku Murata, Shigeru Utsumi, Bunzo Mikami
Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2006, 62(Pt 4);381-4
[PubMed:16582490]
[WorldCat.org]
[DOI]
(I p)
Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221]
[WorldCat.org]
[DOI]
(P p)
