Difference between revisions of "PheT"
| Line 14: | Line 14: | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || [[translation]] | |style="background:#ABCDEF;" align="center"|'''Function''' || [[translation]] | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28630 pheT] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PheT PheT] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PheT PheT] | ||
| Line 26: | Line 26: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnhC]]'', ''[[pheS]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnhC]]'', ''[[pheS]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28630 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28630 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28630 Advanced_DNA] |
|- | |- | ||
|- | |- | ||
Revision as of 13:32, 13 May 2013
- Description: phenylalanyl-tRNA synthetase (beta subunit)
| Gene name | pheT |
| Synonyms | |
| Essential | yes PubMed |
| Product | phenylalanyl-tRNA synthetase (beta subunit) |
| Function | translation |
| Gene expression levels in SubtiExpress: pheT | |
| Interactions involving this protein in SubtInteract: PheT | |
| Metabolic function and regulation of this protein in SubtiPathways: tRNA charging | |
| MW, pI | 87 kDa, 4.864 |
| Gene length, protein length | 2412 bp, 804 aa |
| Immediate neighbours | rnhC, pheS |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU28630
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe) (according to Swiss-Prot)
- Protein family: Type 1 subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- UniProt: P17922
- KEGG entry: [3]
- E.C. number: 6.1.1.20
Additional information
Expression and regulation
- Regulatory mechanism:
- T-box: RNA switch, transcriptional antitermination PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532]
[WorldCat.org]
[DOI]
(I p)
A A Brakhage, M Wozny, H Putzer
Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes.
Biochimie: 1990, 72(10);725-34
[PubMed:2127701]
[WorldCat.org]
[DOI]
(P p)
