Difference between revisions of "NucB"

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|style="background:#ABCDEF;" align="center"|'''Function''' || DNA degradation after mother cell lysis  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || DNA degradation after mother cell lysis  
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU25750 nucB]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25750 nucB]
 
|-
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 5.173   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 5.173   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqeB]]'', ''[[spoIVCB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqeB]]'', ''[[spoIVCB]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14516&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25750 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25750 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25750 Advanced_DNA]
 
|-
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:nucB_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:nucB_context.gif]]

Revision as of 13:22, 13 May 2013

  • Description: sporulation-specific extracellular nuclease

Gene name nucB
Synonyms
Essential no
Product sporulation-specific extracellular nuclease
Function DNA degradation after mother cell lysis
Gene expression levels in SubtiExpress: nucB
MW, pI 14 kDa, 5.173
Gene length, protein length 408 bp, 136 aa
Immediate neighbours yqeB, spoIVCB
Sequences Protein DNA Advanced_DNA
Genetic context
NucB context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

utilization of nucleotides, membrane proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

  • Locus tag: BSU25750

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed during sporulation (SigE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Shigeo Hosoya, Zuolei Lu, Yousuke Ozaki, Michio Takeuchi, Tsutomu Sato
Cytological analysis of the mother cell death process during sporulation in Bacillus subtilis.
J Bacteriol: 2007, 189(6);2561-5
[PubMed:17209033] [WorldCat.org] [DOI] (P p)

D van Sinderen, R Kiewiet, G Venema
Differential expression of two closely related deoxyribonuclease genes, nucA and nucB, in Bacillus subtilis.
Mol Microbiol: 1995, 15(2);213-23
[PubMed:7746143] [WorldCat.org] [DOI] (P p)