Difference between revisions of "SpoIVB"
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|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[SigK]] activation | |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[SigK]] activation | ||
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− | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24230 spoIVB] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SpoIVB SpoIVB] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SpoIVB SpoIVB] | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spo0A]]'', ''[[recN]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spo0A]]'', ''[[recN]]'' | ||
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− | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU24230 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU24230 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU24230 Advanced_DNA] |
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Revision as of 13:15, 13 May 2013
- Description: serine protease, cleaves SpoIVFA, this results in pro-SigK processing/activation in the mother-cell, does also cleave CtpB
Gene name | spoIVB |
Synonyms | |
Essential | no |
Product | serine protease |
Function | control of SigK activation |
Gene expression levels in SubtiExpress: spoIVB | |
Interactions involving this protein in SubtInteract: SpoIVB | |
MW, pI | 45 kDa, 8.842 |
Gene length, protein length | 1275 bp, 425 aa |
Immediate neighbours | spo0A, recN |
Sequences | Protein DNA Advanced_DNA |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
sigma factors and their control, sporulation proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU24230
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Self-cleaves 52-Val-|-Asn-53, 62-Ala-|-Phe-63 and 74-Val-|-Thr-75 at the N-terminus of SpoIVB (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P17896
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: spoIVB PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Simon Cutting, London, UK homepage
Your additional remarks
References
Nathalie Campo, David Z Rudner
SpoIVB and CtpB are both forespore signals in the activation of the sporulation transcription factor sigmaK in Bacillus subtilis.
J Bacteriol: 2007, 189(16);6021-7
[PubMed:17557826]
[WorldCat.org]
[DOI]
(P p)
Thierry Doan, David Z Rudner
Perturbations to engulfment trigger a degradative response that prevents cell-cell signalling during sporulation in Bacillus subtilis.
Mol Microbiol: 2007, 64(2);500-11
[PubMed:17493131]
[WorldCat.org]
[DOI]
(P p)
Nathalie Campo, David Z Rudner
A branched pathway governing the activation of a developmental transcription factor by regulated intramembrane proteolysis.
Mol Cell: 2006, 23(1);25-35
[PubMed:16818230]
[WorldCat.org]
[DOI]
(P p)
Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325]
[WorldCat.org]
[DOI]
(P p)
Tran C Dong, Simon M Cutting
The PDZ domain of the SpoIVB transmembrane signaling protein enables cis-trans interactions involving multiple partners leading to the activation of the pro-sigmaK processing complex in Bacillus subtilis.
J Biol Chem: 2004, 279(42);43468-78
[PubMed:15292188]
[WorldCat.org]
[DOI]
(P p)
Qi Pan, Richard Losick, David Z Rudner
A second PDZ-containing serine protease contributes to activation of the sporulation transcription factor sigmaK in Bacillus subtilis.
J Bacteriol: 2003, 185(20);6051-6
[PubMed:14526016]
[WorldCat.org]
[DOI]
(P p)
Tran C Dong, Simon M Cutting
SpoIVB-mediated cleavage of SpoIVFA could provide the intercellular signal to activate processing of Pro-sigmaK in Bacillus subtilis.
Mol Microbiol: 2003, 49(5);1425-34
[PubMed:12940997]
[WorldCat.org]
[DOI]
(P p)
Ngo T Hoa, James A Brannigan, Simon M Cutting
The Bacillus subtilis signaling protein SpoIVB defines a new family of serine peptidases.
J Bacteriol: 2002, 184(1);191-9
[PubMed:11741860]
[WorldCat.org]
[DOI]
(P p)
N T Hoa, J A Brannigan, S M Cutting
The PDZ domain of the SpoIVB serine peptidase facilitates multiple functions.
J Bacteriol: 2001, 183(14);4364-73
[PubMed:11418578]
[WorldCat.org]
[DOI]
(P p)
P Wakeley, N T Hoa, S Cutting
BofC negatively regulates SpoIVB-mediated signalling in the Bacillus subtilis sigmaK-checkpoint.
Mol Microbiol: 2000, 36(6);1415-24
[PubMed:10931291]
[WorldCat.org]
[DOI]
(P p)
P R Wakeley, R Dorazi, N T Hoa, J R Bowyer, S M Cutting
Proteolysis of SpolVB is a critical determinant in signalling of Pro-sigmaK processing in Bacillus subtilis.
Mol Microbiol: 2000, 36(6);1336-48
[PubMed:10931284]
[WorldCat.org]
[DOI]
(P p)
M Gomez, S M Cutting
Expression of the Bacillus subtilis spoIVB gene is under dual sigma F/sigma G control.
Microbiology (Reading): 1996, 142 ( Pt 12);3453-7
[PubMed:9004507]
[WorldCat.org]
[DOI]
(P p)
I Bagyan, J Hobot, S Cutting
A compartmentalized regulator of developmental gene expression in Bacillus subtilis.
J Bacteriol: 1996, 178(15);4500-7
[PubMed:8755877]
[WorldCat.org]
[DOI]
(P p)
S Cutting, A Driks, R Schmidt, B Kunkel, R Losick
Forespore-specific transcription of a gene in the signal transduction pathway that governs Pro-sigma K processing in Bacillus subtilis.
Genes Dev: 1991, 5(3);456-66
[PubMed:1900494]
[WorldCat.org]
[DOI]
(P p)
B E Van Hoy, J A Hoch
Characterization of the spoIVB and recN loci of Bacillus subtilis.
J Bacteriol: 1990, 172(3);1306-11
[PubMed:2106508]
[WorldCat.org]
[DOI]
(P p)