Difference between revisions of "MifM"

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|style="background:#ABCDEF;" align="center"|'''Function''' || control of ''[[yidC2]]'' translation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || control of ''[[yidC2]]'' translation
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU23880 mifM]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23880 mifM]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/MifM MifM]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/MifM MifM]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[polY1]]'', ''[[yidC2]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[polY1]]'', ''[[yidC2]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAE01460&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23880 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23880 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23880 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yqzJ_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yqzJ_context.gif]]

Revision as of 13:14, 13 May 2013

  • Description: ribosome-nascent chain sensor of membrane protein biogenesis

Gene name mifM
Synonyms yqzJ
Essential no
Product sensor of SpoIIIJ activity
Function control of yidC2 translation
Gene expression levels in SubtiExpress: mifM
Interactions involving this protein in SubtInteract: MifM
MW, pI 11 kDa, 5.829
Gene length, protein length 285 bp, 95 aa
Immediate neighbours polY1, yidC2
Sequences Protein DNA Advanced_DNA
Genetic context
YqzJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MifM expression.png















Categories containing this gene/protein

translation, membrane proteins

This gene is a member of the following regulons

The MifM regulon: yidC2

The gene

Basic information

  • Locus tag: BSU23880

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: acts as a kind of "leader peptide" that does or does not allow translation through a hairpin structure in the yidC2 mRNA PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • N-terminal transmembrane domain PubMed
    • the C-terminal domain interacts with the ribosome and arrests the own translation elongation PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • An antisense RNA is predicted for mifM PubMed
    • MifM has intrinsic sequences to arrest ribosome function, the arrest is influenced by the cotranslational engagement in the protein localization reaction PubMed
    • elongation proceeds when SpoIIIJ integrates the nascent chain of MifM into the membrane PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References