Difference between revisions of "Hbs"

From SubtiWiki
Jump to: navigation, search
Line 14: Line 14:
 
|style="background:#ABCDEF;" align="center"|'''Function''' || DNA packaging, function of the signal recognition complex
 
|style="background:#ABCDEF;" align="center"|'''Function''' || DNA packaging, function of the signal recognition complex
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU22790 hbs]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22790 hbs]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 9 kDa, 9.501   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 9 kDa, 9.501   
Line 22: Line 22:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[folE]]'', ''[[spoIVA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[folE]]'', ''[[spoIVA]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14195&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22790 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22790 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22790 Advanced_DNA]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:hbs_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:hbs_context.gif]]

Revision as of 13:09, 13 May 2013

  • Description: non-specific DNA-binding protein Hbsu

Gene name hbs
Synonyms dbpA, hupA
Essential yes PubMed
Product non-specific DNA-binding protein Hbsu
Function DNA packaging, function of the signal recognition complex
Gene expression levels in SubtiExpress: hbs
MW, pI 9 kDa, 9.501
Gene length, protein length 276 bp, 92 aa
Immediate neighbours folE, spoIVA
Sequences Protein DNA Advanced_DNA
Genetic context
Hbs context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Hbs expression.png















Categories containing this gene/protein

DNA condensation/ segregation, essential genes, membrane proteins, phosphoproteins

This gene is a member of the following regulons

LexA regulon, SigH regulon

The gene

Basic information

  • Locus tag: BSU22790

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • Hbs-mediated untwisting of the DNA stimulates Nth endonuclease activity at AP sites PubMed
  • Protein family: DbpA subfamily (according to Swiss-Prot)
  • Paralogous protein(s): YonN

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-61 PubMed
    • phosphorylation on Thr-4 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1HUU (Geobacillus stearothermophilus)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced by DNA damage (LexA) PubMed
    • strongly repressed in response to glucose starvation in M9 medium PubMed
  • Regulatory mechanism:
  • Additional information:
    • mRNA is protected from degradation by RnjA by initiating ribosomes PubMed
    • Hbs is present with about 50,000 copies per genome in vegetative cells and spores, it is one of the most abundant proteins in B. subtilis PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Wolfgang Klein, Mohamed A Marahiel
Structure-function relationship and regulation of two Bacillus subtilis DNA-binding proteins, HBsu and AbrB.
J Mol Microbiol Biotechnol: 2002, 4(3);323-9
[PubMed:11931565] [WorldCat.org] (P p)

Original publications

Additional publications: PubMed