Difference between revisions of "Des"

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|style="background:#ABCDEF;" align="center"|'''Function''' || adaptation of membrane fluidity at low temperatures
 
|style="background:#ABCDEF;" align="center"|'''Function''' || adaptation of membrane fluidity at low temperatures
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU19180 des]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU19180 des]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/Des Des]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/Des Des]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yocD]]'', ''[[desK]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yocD]]'', ''[[desK]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13810&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU19180 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU19180 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU19180 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:des_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:des_context.gif]]

Revision as of 13:00, 13 May 2013

  • Description: phospholipid desaturase

Gene name des
Synonyms yocE
Essential no
Product phospholipid desaturase
Function adaptation of membrane fluidity at low temperatures
Gene expression levels in SubtiExpress: des
Interactions involving this protein in SubtInteract: Des
Metabolic function and regulation of this protein in SubtiPathways:
Fatty acid degradation
MW, pI 40 kDa, 9.88
Gene length, protein length 1056 bp, 352 aa
Immediate neighbours yocD, desK
Sequences Protein DNA Advanced_DNA
Genetic context
Des context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Des expression.png




























Categories containing this gene/protein

lipid metabolism/ other, cold stress proteins, membrane proteins

This gene is a member of the following regulons

DesR regulon

The gene

Basic information

  • Locus tag: BSU19180

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

    • in strains evolved for growth at low pressure, des expression is significantly increased as compared to the wild type, this allows growth at low pressure PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: introduces double bonds into long chain fatty acids PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
    • Des receives electrons from ferredoxin (Fer) or from either of the two flavodoxins, YkuN or YkuP, the three proteins can functionally replace each other PubMed
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced upon cold shock (DesR) PubMed
    • repressed at increased membrane fluidity PubMed
  • Additional information:
    • in strains evolved for growth at low pressure, des expression is significantly increased as compared to the wild type PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed