Difference between revisions of "SpoIISA"
| Line 13: | Line 13: | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || programmed cell death | |style="background:#ABCDEF;" align="center"|'''Function''' || programmed cell death | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU12830 spoIISA] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SpoIISA SpoIISA] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SpoIISA SpoIISA] | ||
| Line 23: | Line 23: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoIISB]]'', ''[[pit]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoIISB]]'', ''[[pit]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU12830 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU12830 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU12830 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:spoIISA_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:spoIISA_context.gif]] | ||
Revision as of 12:37, 13 May 2013
- Description: lethal when synthesized during vegetative growth in the absence of SpoIISB
| Gene name | spoIISA |
| Synonyms | ykaC |
| Essential | no |
| Product | toxin |
| Function | programmed cell death |
| Gene expression levels in SubtiExpress: spoIISA | |
| Interactions involving this protein in SubtInteract: SpoIISA | |
| MW, pI | 28 kDa, 4.683 |
| Gene length, protein length | 744 bp, 248 aa |
| Immediate neighbours | spoIISB, pit |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
sporulation proteins, toxins, antitoxins and immunity against toxins, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU12830
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains: three putative membrane-spanning segments and a cytoplasmic domain
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane PubMed
Database entries
- UniProt: O34853
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- levels increase at the point of entry into stationary phase of cell cultures grown in sporulation-inducing medium, this is independent of Spo0A PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Patrik Florek, Vladimir M Levdikov, Elena Blagova, Andrey A Lebedev, Rostislav Škrabana, Stanislava Resetárová, Pamela Pavelcíková, Imrich Barak, Anthony J Wilkinson
The structure and interactions of SpoIISA and SpoIISB, a toxin-antitoxin system in Bacillus subtilis.
J Biol Chem: 2011, 286(8);6808-19
[PubMed:21147767]
[WorldCat.org]
[DOI]
(I p)
Patrik Florek, Katarína Muchová, Pamela Pavelcíková, Imrich Barák
Expression of functional Bacillus SpoIISAB toxin-antitoxin modules in Escherichia coli.
FEMS Microbiol Lett: 2008, 278(2);177-84
[PubMed:18096016]
[WorldCat.org]
[DOI]
(P p)
E Adler, I Barák, P Stragier
Bacillus subtilis locus encoding a killer protein and its antidote.
J Bacteriol: 2001, 183(12);3574-81
[PubMed:11371520]
[WorldCat.org]
[DOI]
(P p)
Additional Publications: PubMed
