Difference between revisions of "LcfB"
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|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid degradation | |style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid degradation | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10270 lcfB] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_deg.html Fatty acid degradation]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_deg.html Fatty acid degradation]''' | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhfK]]'', ''[[yhfM]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhfK]]'', ''[[yhfM]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10270 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10270 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10270 Advanced_DNA] |
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Revision as of 12:28, 13 May 2013
- Description: long-chain fatty-acid-CoA ligase, involved in surfactin production
| Gene name | lcfB |
| Synonyms | yhfL |
| Essential | no |
| Product | long-chain fatty-acid-CoA ligase |
| Function | fatty acid degradation |
| Gene expression levels in SubtiExpress: lcfB | |
| Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation | |
| MW, pI | 56 kDa, 5.301 |
| Gene length, protein length | 1539 bp, 513 aa |
| Immediate neighbours | yhfK, yhfM |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU10270
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis PubMed
- Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O07610
- KEGG entry: [3]
- E.C. number: 6.2.1.3
Additional information
Expression and regulation
- Operon: lcfB PubMed
- Regulation:
- induced in the presence of long-chain fatty acids (FadR) PubMed
- subject to carbon catabolite repression (CcpA-HPr(Ser-P) PubMed
- Regulatory mechanism:
- FadR: transcription repression PubMed
- CcpA-HPr(Ser-P): transcription repression PubMed
- Additional information:
Biological materials
- Mutant: available in Mohamed Marahiel's lab PubMed
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
