Difference between revisions of "GroEL"

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|style="background:#ABCDEF;" align="center"|'''Function''' || protein folding and re-folding  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || protein folding and re-folding  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU06030 groEL]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06030 groEL]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/GroEL GroEL]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/GroEL GroEL]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[groES]]'', ''[[ydzT/1]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[groES]]'', ''[[ydzT/1]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12422&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU06030 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU06030 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU06030 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:groEL_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:groEL_context.gif]]

Revision as of 12:16, 13 May 2013

  • Description: chaperonin and co-repressor for HrcA

Gene name groEL
Synonyms
Essential yes PubMed
Product chaperonin, co-repressor for HrcA
Function protein folding and re-folding
Gene expression levels in SubtiExpress: groEL
Interactions involving this protein in SubtInteract: GroEL
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 57 kDa, 4.531
Gene length, protein length 1632 bp, 544 aa
Immediate neighbours groES, ydzT/1
Sequences Protein DNA Advanced_DNA
Genetic context
GroEL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GroEL expression.png




























Categories containing this gene/protein

chaperones/ protein folding, heat shock proteins, essential genes, phosphoproteins

This gene is a member of the following regulons

HrcA regulon

The gene

Basic information

  • Locus tag: BSU06030

Expression

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: acts as co-repressor for HrcA
  • Protein family: chaperonin (HSP60) family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-35, Arg-116, and Arg-282 PubMed
    • phosphorylated on ser/ thr/ tyr PubMed, PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • cytoplasm (according to Swiss-Prot)
    • recruited to the membrane after ethanol stress PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism: HrcA: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1000 available in Stülke lab
    • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP1002 available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage

Your additional remarks

References

Additional publications: PubMed