Difference between revisions of "NasF"

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|style="background:#ABCDEF;" align="center"|'''Function''' || nitrate respiration  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || nitrate respiration  
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU03280 nasF]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03280 nasF]
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/other_nitrogen_sources/index.html Alternative nitrogen sources]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/other_nitrogen_sources/index.html Alternative nitrogen sources]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ycgT]]'', ''[[nasE]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ycgT]]'', ''[[nasE]]''
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12122&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU03280 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU03280 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU03280 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:nasF_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:nasF_context.gif]]

Revision as of 12:06, 13 May 2013

  • Description: uroporphyrinogen methyltransferase

Gene name nasF
Synonyms nasBE
Essential no
Product uroporphyrinogen methyltransferase
Function nitrate respiration
Gene expression levels in SubtiExpress: nasF
Metabolic function and regulation of this protein in SubtiPathways:
Alternative nitrogen sources
MW, pI 53 kDa, 6.523
Gene length, protein length 1449 bp, 483 aa
Immediate neighbours ycgT, nasE
Sequences Protein DNA Advanced_DNA
Genetic context
NasF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NasF expression.png
























Categories containing this gene/protein

respiration, utilization of nitrogen sources other than amino acids

This gene is a member of the following regulons

Fur regulon, NsrR regulon, ResD regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU03280

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1 (according to Swiss-Prot)
  • Protein family: precorrin methyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • expressed under anaerobic conditions (ResD) PubMed
    • induced by nitric oxide under anaerobic conditions (NsrR) PubMed
    • induced by iron starvation (Fur) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Víctor M Luque-Almagro, Andrew J Gates, Conrado Moreno-Vivián, Stuart J Ferguson, David J Richardson, M Dolores Roldán
Bacterial nitrate assimilation: gene distribution and regulation.
Biochem Soc Trans: 2011, 39(6);1838-43
[PubMed:22103536] [WorldCat.org] [DOI] (I p)

Original publications