Difference between revisions of "CwlJ"
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|style="background:#ABCDEF;" align="center"|'''Function''' || spore germination | |style="background:#ABCDEF;" align="center"|'''Function''' || spore germination | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU02600 cwlJ] |
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 9.354 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 9.354 | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ycbP]]'', ''[[ycbR]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ycbP]]'', ''[[ycbR]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU02600 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU02600 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU02600 Advanced_DNA] |
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Revision as of 12:04, 13 May 2013
- Description: cell wall hydrolase
| Gene name | cwlJ |
| Synonyms | ycbQ |
| Essential | no |
| Product | cell wall hydrolase |
| Function | spore germination |
| Gene expression levels in SubtiExpress: cwlJ | |
| MW, pI | 16 kDa, 9.354 |
| Gene length, protein length | 426 bp, 142 aa |
| Immediate neighbours | ycbP, ycbR |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell wall/ other, sporulation proteins, germination
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU02600
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: cwlJ family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- outer edge of the spore cortex PubMed
Database entries
- Structure:
- UniProt: P42249
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Yunfeng Li, Xuan Y Butzin, Andrew Davis, Barbara Setlow, George Korza, Fatma Işik Üstok, Graham Christie, Peter Setlow, Bing Hao
Activity and regulation of various forms of CwlJ, SleB, and YpeB proteins in degrading cortex peptidoglycan of spores of Bacillus species in vitro and during spore germination.
J Bacteriol: 2013, 195(11);2530-40
[PubMed:23543708]
[WorldCat.org]
[DOI]
(I p)
Xuan Yi, Peter Setlow
Studies of the commitment step in the germination of spores of bacillus species.
J Bacteriol: 2010, 192(13);3424-33
[PubMed:20435722]
[WorldCat.org]
[DOI]
(I p)
Alicia Monroe, Peter Setlow
Localization of the transglutaminase cross-linking sites in the Bacillus subtilis spore coat protein GerQ.
J Bacteriol: 2006, 188(21);7609-16
[PubMed:16936016]
[WorldCat.org]
[DOI]
(P p)
Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190]
[WorldCat.org]
[DOI]
(P p)
Katerina Ragkousi, Peter Setlow
Transglutaminase-mediated cross-linking of GerQ in the coats of Bacillus subtilis spores.
J Bacteriol: 2004, 186(17);5567-75
[PubMed:15317760]
[WorldCat.org]
[DOI]
(P p)
Haridasan Chirakkal, Michele O'Rourke, Abdelmadjid Atrih, Simon J Foster, Anne Moir
Analysis of spore cortex lytic enzymes and related proteins in Bacillus subtilis endospore germination.
Microbiology (Reading): 2002, 148(Pt 8);2383-2392
[PubMed:12177332]
[WorldCat.org]
[DOI]
(P p)
Irina Bagyan, Peter Setlow
Localization of the cortex lytic enzyme CwlJ in spores of Bacillus subtilis.
J Bacteriol: 2002, 184(4);1219-24
[PubMed:11807087]
[WorldCat.org]
[DOI]
(P p)
M Paidhungat, K Ragkousi, P Setlow
Genetic requirements for induction of germination of spores of Bacillus subtilis by Ca(2+)-dipicolinate.
J Bacteriol: 2001, 183(16);4886-93
[PubMed:11466292]
[WorldCat.org]
[DOI]
(P p)
S Ishikawa, K Yamane, J Sekiguchi
Regulation and characterization of a newly deduced cell wall hydrolase gene (cwlJ) which affects germination of Bacillus subtilis spores.
J Bacteriol: 1998, 180(6);1375-80
[PubMed:9515903]
[WorldCat.org]
[DOI]
(P p)
